Matches in SemOpenAlex for { <https://semopenalex.org/work/W2035490786> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2035490786 endingPage "78" @default.
- W2035490786 startingPage "72" @default.
- W2035490786 abstract "When the 34 kDa kinase domain of human spleen tyrosine kinase (Syk-KD) was expressed as a C-terminally His-tagged protein in baculovirus-infected Sf-21 insect cells, the purified protein included two forms that migrated slightly differently in SDS-polyacrylamide gel electrophoresis. Intact mass analysis and LC-MS/MS peptide mapping showed that the major and faster-migrating product had the intended amino-acid sequence and 0-6 phosphorylations. This material accounted for about 95% of the purified protein. The minor product was Syk-KD with a 26 amino-acid N-terminal extension. The result suggested the existence of an upstream alternative site for the initiation of translation, and this proved to be an ACG codon derived from the pBacPAK9 vector used to express Syk-KD. The ACG codon was preceded and followed by Kozak-type sequence elements (a purine in the -3 position and a G in the +4 position) that would have enhanced the viability of initiation at ACG. The initiating amino-acid residue was Met for both minor and major products, and both forms of the protein were α-N-acetylated. For the minor product, protein intact mass analysis and peptide mapping both gave results in agreement with the sequence predicted from the DNA. A similar result with the same underlying cause was obtained with insect cell expression of full-length Syk. It appears that similar results are possible whenever this vector is used." @default.
- W2035490786 created "2016-06-24" @default.
- W2035490786 creator A5015039185 @default.
- W2035490786 creator A5038351786 @default.
- W2035490786 creator A5051964695 @default.
- W2035490786 creator A5053366049 @default.
- W2035490786 creator A5062835519 @default.
- W2035490786 creator A5078013894 @default.
- W2035490786 creator A5086265496 @default.
- W2035490786 date "2011-03-01" @default.
- W2035490786 modified "2023-09-26" @default.
- W2035490786 title "Initiation of translation at an upstream non-AUG codon accounting for N-terminally extended minor forms of recombinant proteins expressed in insect cells" @default.
- W2035490786 cites W1536042412 @default.
- W2035490786 cites W1558507319 @default.
- W2035490786 cites W1973525252 @default.
- W2035490786 cites W1981593008 @default.
- W2035490786 cites W1981978551 @default.
- W2035490786 cites W1999139162 @default.
- W2035490786 cites W2002609852 @default.
- W2035490786 cites W2005633684 @default.
- W2035490786 cites W2020828649 @default.
- W2035490786 cites W2025642590 @default.
- W2035490786 cites W2037313242 @default.
- W2035490786 cites W2043986644 @default.
- W2035490786 cites W2058426472 @default.
- W2035490786 cites W2063004754 @default.
- W2035490786 cites W2083070222 @default.
- W2035490786 cites W2085843814 @default.
- W2035490786 cites W2139260526 @default.
- W2035490786 cites W2162658917 @default.
- W2035490786 cites W2175553381 @default.
- W2035490786 cites W2176788572 @default.
- W2035490786 cites W4245861869 @default.
- W2035490786 doi "https://doi.org/10.1016/j.pep.2010.09.015" @default.
- W2035490786 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20888915" @default.
- W2035490786 hasPublicationYear "2011" @default.
- W2035490786 type Work @default.
- W2035490786 sameAs 2035490786 @default.
- W2035490786 citedByCount "4" @default.
- W2035490786 countsByYear W20354907862013 @default.
- W2035490786 countsByYear W20354907862015 @default.
- W2035490786 crossrefType "journal-article" @default.
- W2035490786 hasAuthorship W2035490786A5015039185 @default.
- W2035490786 hasAuthorship W2035490786A5038351786 @default.
- W2035490786 hasAuthorship W2035490786A5051964695 @default.
- W2035490786 hasAuthorship W2035490786A5053366049 @default.
- W2035490786 hasAuthorship W2035490786A5062835519 @default.
- W2035490786 hasAuthorship W2035490786A5078013894 @default.
- W2035490786 hasAuthorship W2035490786A5086265496 @default.
- W2035490786 hasConcept C104317684 @default.
- W2035490786 hasConcept C105580179 @default.
- W2035490786 hasConcept C153911025 @default.
- W2035490786 hasConcept C167625842 @default.
- W2035490786 hasConcept C176990463 @default.
- W2035490786 hasConcept C2779281246 @default.
- W2035490786 hasConcept C35174551 @default.
- W2035490786 hasConcept C42362537 @default.
- W2035490786 hasConcept C515207424 @default.
- W2035490786 hasConcept C55493867 @default.
- W2035490786 hasConcept C62478195 @default.
- W2035490786 hasConcept C86803240 @default.
- W2035490786 hasConceptScore W2035490786C104317684 @default.
- W2035490786 hasConceptScore W2035490786C105580179 @default.
- W2035490786 hasConceptScore W2035490786C153911025 @default.
- W2035490786 hasConceptScore W2035490786C167625842 @default.
- W2035490786 hasConceptScore W2035490786C176990463 @default.
- W2035490786 hasConceptScore W2035490786C2779281246 @default.
- W2035490786 hasConceptScore W2035490786C35174551 @default.
- W2035490786 hasConceptScore W2035490786C42362537 @default.
- W2035490786 hasConceptScore W2035490786C515207424 @default.
- W2035490786 hasConceptScore W2035490786C55493867 @default.
- W2035490786 hasConceptScore W2035490786C62478195 @default.
- W2035490786 hasConceptScore W2035490786C86803240 @default.
- W2035490786 hasIssue "1" @default.
- W2035490786 hasLocation W20354907861 @default.
- W2035490786 hasLocation W20354907862 @default.
- W2035490786 hasOpenAccess W2035490786 @default.
- W2035490786 hasPrimaryLocation W20354907861 @default.
- W2035490786 hasRelatedWork W1495028395 @default.
- W2035490786 hasRelatedWork W1993579736 @default.
- W2035490786 hasRelatedWork W1995237641 @default.
- W2035490786 hasRelatedWork W1995278782 @default.
- W2035490786 hasRelatedWork W1995523102 @default.
- W2035490786 hasRelatedWork W2016062340 @default.
- W2035490786 hasRelatedWork W2020644290 @default.
- W2035490786 hasRelatedWork W2022334616 @default.
- W2035490786 hasRelatedWork W2189303013 @default.
- W2035490786 hasRelatedWork W3174912450 @default.
- W2035490786 hasVolume "76" @default.
- W2035490786 isParatext "false" @default.
- W2035490786 isRetracted "false" @default.
- W2035490786 magId "2035490786" @default.
- W2035490786 workType "article" @default.