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• W2035526103 abstract "Streptococcus suis, a Gram-positive coccus, is an emerging zoonotic pathogen for both humans and pigs, but little is known about the properties of its metabolic enzymes. Isocitrate dehydrogenase (IDH) is a key regulatory enzyme in the citric acid cycle that catalyzes the oxidative decarboxylation of isocitrate yielding α-ketoglutarate and NAD(P)H. Here, we report the overexpression and enzymatic characterization of IDH from S. suis Serotype 2 Chinese highly virulent strain 05ZYH33 (SsIDH). The molecular weight of SsIDH was estimated to be 74 kDa by gel filtration chromatography, suggesting a homodimeric structure. Additionally, SsIDH was divalent cation-dependent and Mg(2+) was found to be the most effective cation. The optimal pH of SsIDH was 7.0 (Mn(2+)) and 8.5 (Mg(2+)), and the maximum activity was around 30 °C (Mn(2+)) and 50 °C (Mg(2+)), respectively. Heat inactivation studies showed that SsIDH retained 50% activity after 20 min of incubation at 49 °C. Sequence comparison revealed that SsIDH had a significantly homologous identity to bacterial homodimeric IDHs. The recombinant SsIDH displayed a 117-fold (k(cat)/K(m)) preference for NAD(+) over NADP(+) with Mg(2+), and a 80-fold greater specificity for NAD(+) than NADP(+) with Mn(2+). Therefore, SsIDH has remarkably high coenzyme preference toward NAD(+). This current work is expected to shed light on the functions of metabolic enzymes in S. suis and provide useful information for SsIDH to be considered as a possible candidate for serological diagnostics and detection of S. suis infection." @default.
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• W2035526103 date "2011-09-01" @default.
• W2035526103 modified "2023-10-06" @default.
• W2035526103 title "Enzymatic characterization of isocitrate dehydrogenase from an emerging zoonotic pathogen Streptococcus suis" @default.
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• W2035526103 doi "https://doi.org/10.1016/j.biochi.2011.04.021" @default.
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