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• W2035537757 abstract "Phloridzin (phloretin 2′-O-glucoside) is the most abundant phenolic compound in apple (Malus x domestica) and represents up to 14% of dry weight in leaves. In contrast, the closely related genus pear (Pyrus sp.) does not accumulate any phloretin derivatives. Glycosylation of phloretin at position 2′ is the last step in the biosynthesis of phloridzin. Using a PCR-based cloning strategy we cloned and characterised five novel glycosyltransferases with low amino acid sequence identities (34.1–46.3%) from apple (clones UGT71A15 and UGT71K1) and pear (clones UGT71A16, UGT71K2 and UGT88F2), which are able to glycosylate phloretin to phloridzin in vitro. Additionally we identified phloretin 4′-O-glucoside and phloretin 4-O-glucoside as by-products of the apple clone UGT71A15. The occurrence and NMR data of phloretin 4-O-glucoside are described for the first time. With the exception of the pear clone UGT88F2, which is orthologous to a known uridine diphosphoglucose:phloretin 2′-O-glycosyltransferase from apple (UGT88F1), all clones were able to glycosylate several phenolic substrates other than phloretin. We show that different glycosyltransferases from M. x domestica could be responsible for phloridzin formation in planta and that Pyrus communis has the ability to glycosylate phloretin, although no phloretin derivatives are present in this genus." @default.
• W2035537757 created "2016-06-24" @default.
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• W2035537757 date "2010-03-01" @default.
• W2035537757 modified "2023-09-28" @default.
• W2035537757 title "Cloning and heterologous expression of glycosyltransferases from Malus x domestica and Pyrus communis, which convert phloretin to phloretin 2′-O-glucoside (phloridzin)" @default.
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• W2035537757 doi "https://doi.org/10.1016/j.plantsci.2009.12.009" @default.
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