FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2035561323> ?p ?o ?g. }

• W2035561323 endingPage "211" @default.
• W2035561323 startingPage "203" @default.
• W2035561323 abstract "Most of the important forage and turf grasses are outbreeders, require vernalization to flower, and in some cases are polyploid. With the development of gene sequencing information in grasses, there is an urgent need for a model system to perform large-scale functional analysis of candidate genes. We propose to use Lolium temulentum L. (Darnel ryegrass) as a model system for genetic manipulation studies in forage and turf grasses because L. temulentum has the following advantages: self-fertile, short life cycle (11 weeks), diploid, easy to grow, and closely related with other major grass species. In order to improve tissue culture response of L. temulentum, two relatively responsive lines were crossed and putative hybrid seeds obtained. Analysis of the F1 plants by SSR markers confirmed that the F1 plants were true hybrids. Anthers were dissected from F2 plants of the cross, 3.0% of the cultured anthers responded with the formation of calluses. Green haploid plants were recovered from 48.9% of the anther-derived calluses. Seeds were harvested from doubled haploid plants and mature embryos were used as explants for comparing tissue culture responses with other lines. Besides anther culture, F2 seeds of the cross were subjected to a cycle of selection for callus formation and plant regeneration, and F4 seeds were obtained from the regenerated plants. Comparison of tissue culture response of different lines revealed that the doubled haploid plants had a much higher frequency of embryogenic callus formation than that of the parental lines and the F4 seeds. This is the first report on the generation of green haploid and doubled haploid plants in L. temulentum. The technique was successfully used for the rapid production of homozygous diploid plants that are highly tissue culture responsive. The anther culture-derived new L. temulentum lines could be valuable material for functional test of genes in grasses." @default.
• W2035561323 created "2016-06-24" @default.
• W2035561323 creator A5008258073 @default.
• W2035561323 creator A5025971349 @default.
• W2035561323 creator A5030126343 @default.
• W2035561323 creator A5054531448 @default.
• W2035561323 date "2005-01-01" @default.
• W2035561323 modified "2023-10-02" @default.
• W2035561323 title "Development of highly tissue culture responsive lines of Lolium temulentum by anther culture" @default.
• W2035561323 cites W1547733320 @default.
• W2035561323 cites W1597993741 @default.
• W2035561323 cites W1967118561 @default.
• W2035561323 cites W1968672103 @default.
• W2035561323 cites W1982572603 @default.
• W2035561323 cites W1992400845 @default.
• W2035561323 cites W1997796029 @default.
• W2035561323 cites W2000907899 @default.
• W2035561323 cites W2001451873 @default.
• W2035561323 cites W2009197862 @default.
• W2035561323 cites W2010979146 @default.
• W2035561323 cites W2017843187 @default.
• W2035561323 cites W2018728474 @default.
• W2035561323 cites W2021160303 @default.
• W2035561323 cites W2021599793 @default.
• W2035561323 cites W2024556021 @default.
• W2035561323 cites W2026710773 @default.
• W2035561323 cites W2040230788 @default.
• W2035561323 cites W2041360285 @default.
• W2035561323 cites W2044704050 @default.
• W2035561323 cites W2045998325 @default.
• W2035561323 cites W2048641070 @default.
• W2035561323 cites W2053788591 @default.
• W2035561323 cites W2059379600 @default.
• W2035561323 cites W2068555835 @default.
• W2035561323 cites W2071464634 @default.
• W2035561323 cites W2080917431 @default.
• W2035561323 cites W2081486636 @default.
• W2035561323 cites W2086621973 @default.
• W2035561323 cites W2094438125 @default.
• W2035561323 cites W2094938742 @default.
• W2035561323 cites W2106786471 @default.
• W2035561323 cites W2130972289 @default.
• W2035561323 cites W2147101897 @default.
• W2035561323 cites W2150582511 @default.
• W2035561323 cites W2167220491 @default.
• W2035561323 cites W2478577034 @default.
• W2035561323 cites W2520496282 @default.
• W2035561323 cites W295902452 @default.
• W2035561323 cites W4240314266 @default.
• W2035561323 cites W4253856561 @default.
• W2035561323 doi "https://doi.org/10.1016/j.plantsci.2004.07.036" @default.
• W2035561323 hasPublicationYear "2005" @default.
• W2035561323 type Work @default.
• W2035561323 sameAs 2035561323 @default.
• W2035561323 citedByCount "26" @default.
• W2035561323 countsByYear W20355613232012 @default.
• W2035561323 countsByYear W20355613232016 @default.
• W2035561323 countsByYear W20355613232021 @default.
• W2035561323 crossrefType "journal-article" @default.
• W2035561323 hasAuthorship W2035561323A5008258073 @default.
• W2035561323 hasAuthorship W2035561323A5025971349 @default.
• W2035561323 hasAuthorship W2035561323A5030126343 @default.
• W2035561323 hasAuthorship W2035561323A5054531448 @default.
• W2035561323 hasConcept C104317684 @default.
• W2035561323 hasConcept C143191323 @default.
• W2035561323 hasConcept C202751555 @default.
• W2035561323 hasConcept C2776626444 @default.
• W2035561323 hasConcept C2778659099 @default.
• W2035561323 hasConcept C2780618852 @default.
• W2035561323 hasConcept C29172406 @default.
• W2035561323 hasConcept C34827325 @default.
• W2035561323 hasConcept C46757340 @default.
• W2035561323 hasConcept C48189365 @default.
• W2035561323 hasConcept C54355233 @default.
• W2035561323 hasConcept C55969652 @default.
• W2035561323 hasConcept C59822182 @default.
• W2035561323 hasConcept C7259398 @default.
• W2035561323 hasConcept C86803240 @default.
• W2035561323 hasConcept C90703135 @default.
• W2035561323 hasConcept C9549007 @default.
• W2035561323 hasConceptScore W2035561323C104317684 @default.
• W2035561323 hasConceptScore W2035561323C143191323 @default.
• W2035561323 hasConceptScore W2035561323C202751555 @default.
• W2035561323 hasConceptScore W2035561323C2776626444 @default.
• W2035561323 hasConceptScore W2035561323C2778659099 @default.
• W2035561323 hasConceptScore W2035561323C2780618852 @default.
• W2035561323 hasConceptScore W2035561323C29172406 @default.
• W2035561323 hasConceptScore W2035561323C34827325 @default.
• W2035561323 hasConceptScore W2035561323C46757340 @default.
• W2035561323 hasConceptScore W2035561323C48189365 @default.
• W2035561323 hasConceptScore W2035561323C54355233 @default.
• W2035561323 hasConceptScore W2035561323C55969652 @default.
• W2035561323 hasConceptScore W2035561323C59822182 @default.
• W2035561323 hasConceptScore W2035561323C7259398 @default.
• W2035561323 hasConceptScore W2035561323C86803240 @default.
• W2035561323 hasConceptScore W2035561323C90703135 @default.
• W2035561323 hasConceptScore W2035561323C9549007 @default.
• W2035561323 hasIssue "1" @default.

• next