FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2035565255> ?p ?o ?g. }

• W2035565255 endingPage "200" @default.
• W2035565255 startingPage "189" @default.
• W2035565255 abstract "Summary Drug‐monoclonal antibody conjugates have been evaluated for their specificity and toxicity towards tumour cells in vitro and in vivo ; however, few studies have investigated their mode of entry into cells and mechanism of action. In this study the uptake and toxic effect of three different Methotrexate monoclonal antibody (MTX‐MoAb) conjugates (MTX‐anti‐transferrin receptor (TFR). MTX anti‐Ly‐2·1 and MTX‐anti‐L3T4) were examined and compared with free MTX. It was concluded that MTX and these MTX‐MoAb conjugates gain entry into tumour cells and are processed by different mechanisms, considering the following results: (a) alterations in temperature had a greater effect on the toxicity of MTX‐MoAb than on MTX; in addition, MTX and MTX‐MoAb had different rates of action on cells; (b) the specific MTX transport inhibitor, p‐chloromercuribenzene sulphonate (pCMS), reduced MTX toxicity but had no effect on specific MTX‐MoAb conjugates; (c) the concentration of various ions (Ca 2+ , Mg 2+ and Mn 2+ ) effected the entry of MTX MoAb but had no effect on free MTX. MTX enters by its own carrier mechanism, while MTX‐MoAb conjugates enter by endocytosis with release of MTX at the lysosomal membrane, demonstrated by the ability of chloroquine and NH 4 Cl (which inhibit lysosomal function) to inhibit the action of MTX‐MoAb but not MTX. Therefore, these MTX‐MoAb conjugates are not degraded at the surface but bind to their receptor and then enter the cell by endocytosis as one entity; the MTX‐MoAb conjugates are then degraded within the lysosomes, resulting in the release of free MTX into the cytoplasm where it acts on dihydrofolate reductase (DHFR) to inhibit cell metabolism." @default.
• W2035565255 created "2016-06-24" @default.
• W2035565255 creator A5028909942 @default.
• W2035565255 creator A5054416511 @default.
• W2035565255 creator A5061575707 @default.
• W2035565255 date "1987-04-01" @default.
• W2035565255 modified "2023-10-18" @default.
• W2035565255 title "The mode of action of methotrexate-monoclonal antibody conjugates" @default.
• W2035565255 cites W1502327274 @default.
• W2035565255 cites W1833532864 @default.
• W2035565255 cites W1971565040 @default.
• W2035565255 cites W1975913540 @default.
• W2035565255 cites W2006416369 @default.
• W2035565255 cites W2025486698 @default.
• W2035565255 cites W2036229899 @default.
• W2035565255 cites W2046780772 @default.
• W2035565255 cites W2049348498 @default.
• W2035565255 cites W2066537030 @default.
• W2035565255 cites W2071551486 @default.
• W2035565255 cites W2085544778 @default.
• W2035565255 cites W2088085075 @default.
• W2035565255 cites W2113896518 @default.
• W2035565255 cites W2123299536 @default.
• W2035565255 cites W2127874307 @default.
• W2035565255 cites W2149817638 @default.
• W2035565255 cites W2161106503 @default.
• W2035565255 cites W2603700516 @default.
• W2035565255 cites W4239088562 @default.
• W2035565255 doi "https://doi.org/10.1038/icb.1987.21" @default.
• W2035565255 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3610218" @default.
• W2035565255 hasPublicationYear "1987" @default.
• W2035565255 type Work @default.
• W2035565255 sameAs 2035565255 @default.
• W2035565255 citedByCount "24" @default.
• W2035565255 countsByYear W20355652552012 @default.
• W2035565255 countsByYear W20355652552013 @default.
• W2035565255 countsByYear W20355652552014 @default.
• W2035565255 countsByYear W20355652552015 @default.
• W2035565255 countsByYear W20355652552018 @default.
• W2035565255 countsByYear W20355652552019 @default.
• W2035565255 countsByYear W20355652552020 @default.
• W2035565255 crossrefType "journal-article" @default.
• W2035565255 hasAuthorship W2035565255A5028909942 @default.
• W2035565255 hasAuthorship W2035565255A5054416511 @default.
• W2035565255 hasAuthorship W2035565255A5061575707 @default.
• W2035565255 hasConcept C134306372 @default.
• W2035565255 hasConcept C159654299 @default.
• W2035565255 hasConcept C170493617 @default.
• W2035565255 hasConcept C178790620 @default.
• W2035565255 hasConcept C185592680 @default.
• W2035565255 hasConcept C197336794 @default.
• W2035565255 hasConcept C202751555 @default.
• W2035565255 hasConcept C203014093 @default.
• W2035565255 hasConcept C2776120743 @default.
• W2035565255 hasConcept C2779348387 @default.
• W2035565255 hasConcept C2781059491 @default.
• W2035565255 hasConcept C28005876 @default.
• W2035565255 hasConcept C29730261 @default.
• W2035565255 hasConcept C32264632 @default.
• W2035565255 hasConcept C33923547 @default.
• W2035565255 hasConcept C542903549 @default.
• W2035565255 hasConcept C55493867 @default.
• W2035565255 hasConcept C86803240 @default.
• W2035565255 hasConcept C98274493 @default.
• W2035565255 hasConceptScore W2035565255C134306372 @default.
• W2035565255 hasConceptScore W2035565255C159654299 @default.
• W2035565255 hasConceptScore W2035565255C170493617 @default.
• W2035565255 hasConceptScore W2035565255C178790620 @default.
• W2035565255 hasConceptScore W2035565255C185592680 @default.
• W2035565255 hasConceptScore W2035565255C197336794 @default.
• W2035565255 hasConceptScore W2035565255C202751555 @default.
• W2035565255 hasConceptScore W2035565255C203014093 @default.
• W2035565255 hasConceptScore W2035565255C2776120743 @default.
• W2035565255 hasConceptScore W2035565255C2779348387 @default.
• W2035565255 hasConceptScore W2035565255C2781059491 @default.
• W2035565255 hasConceptScore W2035565255C28005876 @default.
• W2035565255 hasConceptScore W2035565255C29730261 @default.
• W2035565255 hasConceptScore W2035565255C32264632 @default.
• W2035565255 hasConceptScore W2035565255C33923547 @default.
• W2035565255 hasConceptScore W2035565255C542903549 @default.
• W2035565255 hasConceptScore W2035565255C55493867 @default.
• W2035565255 hasConceptScore W2035565255C86803240 @default.
• W2035565255 hasConceptScore W2035565255C98274493 @default.
• W2035565255 hasIssue "2" @default.
• W2035565255 hasLocation W20355652551 @default.
• W2035565255 hasLocation W20355652552 @default.
• W2035565255 hasOpenAccess W2035565255 @default.
• W2035565255 hasPrimaryLocation W20355652551 @default.
• W2035565255 hasRelatedWork W101758766 @default.
• W2035565255 hasRelatedWork W1541110228 @default.
• W2035565255 hasRelatedWork W1967497707 @default.
• W2035565255 hasRelatedWork W1987904720 @default.
• W2035565255 hasRelatedWork W2035565255 @default.
• W2035565255 hasRelatedWork W2042155513 @default.
• W2035565255 hasRelatedWork W2072705032 @default.
• W2035565255 hasRelatedWork W2137682426 @default.
• W2035565255 hasRelatedWork W2315421474 @default.
• W2035565255 hasRelatedWork W2403321970 @default.

• next