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- W2035565591 abstract "Molecular motors are studied in vitro to understand their Biophysics and Cell Biology. Most mechanical studies of myosin and other molecular motors have utilized surface-immobilized motors or filaments, which impact their range of motion. One way of avoiding surface immobilization of the filament and motor is to suspend filaments from fixed supports, giving the motor or the motor-coated cargo unimpeded freedom of motion about its track (Ali et al., Nat Struct Biol. 9:464, 2002). We used dielectrophoresis at 4-12 V, 2 MHz to stretch and suspend actin filaments across a 2 x 7 um2 trench etched between two gold electrodes patterned on a glass slide. Optical tweezers were used to bring a myosin-coated bead into close proximity to a pre-selected, suspended actin filament, facilitating bead attachment to the filament in motility buffer. Using defocused images, the bead's three-dimensional position was tracked as a function of time to obtain its trajectory on the actin. Experiments were carried out with myosin V and myosin X. Both motor proteins followed left-handed helical paths with 1.5 - 2 um pitch. Variants of this technique will enable types of higher complexity found in cells to be addressed with in vitro experiments. We thank Drs. Mitsuo Ikebe and Osamu Sato for the gift of myosin X and the Nano/Bio Interface Center (NSF NSEC DMR-0425780) and the NIH (grant AR26846) for support." @default.
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- W2035565591 date "2009-02-01" @default.
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- W2035565591 title "A Novel Method For Investigating The Azimuthal Rotation Of Molecular Motors Utilizing Dielectrophoresis And Optical Tweezers" @default.
- W2035565591 doi "https://doi.org/10.1016/j.bpj.2008.12.1432" @default.
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