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- W2035581662 abstract "Abstract Seven reversed-phase columns, HRPB, Kromasil 100-5-C18, S5ODS2B, Vydac 218TP, YMC Polymer C18, Zorbax SBPhenyl and Zorbax SBCN, were compared with the polymeric Hamilton PRP-1 used so far, for ability to separate and determine imazalil and its degradation product, T824. Zorbax SBCN presented the best performance with sharp peak shape for both compounds, the height for imazalil being much superior to that from the PRP-1 column. It presented the best N and a under the conditions selected and gave a k' of 4.39 and 3.00 for imazalil and T824, respectively. The retention time for T824 was the second greatest of the columns, which is likely to be advantageous for the extracts of interest. As far as time of analysis is concerned, it presented a shorter total time of ca 8 minutes using a ratio of 6:4 for the mobile phase of acetonitrile:water. That ratio was confirmed to give higher and sharper peaks for both compounds and to lead to a lower limit of detection for imazalil and for T824. The better separation obtained for imazalil and T824 with this column is attributed to the reduction of the strong adsorption of basic compounds due to the steric protection of silanol groups. Key Words: HPLCimidazole fungicidesimazalilT824chromatographic separationreversed-phase column" @default.
- W2035581662 created "2016-06-24" @default.
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- W2035581662 date "1995-01-01" @default.
- W2035581662 modified "2023-10-01" @default.
- W2035581662 title "Separation of Imazalil and T824 in Cucurbits—Part one: Comparison of HPLC Reversed-Phase Columns" @default.
- W2035581662 cites W1982331199 @default.
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- W2035581662 doi "https://doi.org/10.1080/03067319508033114" @default.
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