Matches in SemOpenAlex for { <https://semopenalex.org/work/W2035636775> ?p ?o ?g. }
- W2035636775 endingPage "6881" @default.
- W2035636775 startingPage "6873" @default.
- W2035636775 abstract "Members of the Tc1/mariner superfamily of transposable elements isolated from vertebrates are transpositionally inactive due to the accumulation of mutations in their transposase genes. A novel open reading frame-trapping method was used to isolate uninterrupted transposase coding regions from the genome of the frog species Rana pipiens. The isolated clones were approximately 90% identical to a predicted transposase gene sequence from Xenopus laevis, but contained an unpredicted, approximately 180 bp region encoding the N-terminus of the putative transposase. None of these native genes was found to be active. Therefore, a consensus sequence of the transposase gene was derived. This engineered transposase and the transposon inverted repeats together constitute the components of a novel transposon system that we named Frog Prince (FP). FP has only approximately 50% sequence similarity to Sleeping Beauty (SB), and catalyzes efficient cut-and-paste transposition in fish, amphibian and mammalian cell lines. We demonstrate high-efficiency gene trapping in human cells using FP transposition. FP is the most efficient DNA-based transposon from vertebrates described to date, and shows approximately 70% higher activity in zebrafish cells than SB. Frog Prince can greatly extend our possibilities for genetic analyses in vertebrates." @default.
- W2035636775 created "2016-06-24" @default.
- W2035636775 creator A5043517944 @default.
- W2035636775 creator A5052007480 @default.
- W2035636775 creator A5054222559 @default.
- W2035636775 creator A5066552100 @default.
- W2035636775 date "2003-12-01" @default.
- W2035636775 modified "2023-09-26" @default.
- W2035636775 title "The Frog Prince: a reconstructed transposon from Rana pipiens with high transpositional activity in vertebrate cells" @default.
- W2035636775 cites W1495724207 @default.
- W2035636775 cites W1534179616 @default.
- W2035636775 cites W1600900798 @default.
- W2035636775 cites W1695361451 @default.
- W2035636775 cites W1779180564 @default.
- W2035636775 cites W1927506396 @default.
- W2035636775 cites W1932232911 @default.
- W2035636775 cites W1941119369 @default.
- W2035636775 cites W1966486813 @default.
- W2035636775 cites W1971277282 @default.
- W2035636775 cites W1975880129 @default.
- W2035636775 cites W1976006634 @default.
- W2035636775 cites W1977255713 @default.
- W2035636775 cites W1995451465 @default.
- W2035636775 cites W1996001643 @default.
- W2035636775 cites W2001109802 @default.
- W2035636775 cites W2001519016 @default.
- W2035636775 cites W2008490400 @default.
- W2035636775 cites W2013902051 @default.
- W2035636775 cites W2017744445 @default.
- W2035636775 cites W2018442148 @default.
- W2035636775 cites W2026000633 @default.
- W2035636775 cites W2026952154 @default.
- W2035636775 cites W2027120933 @default.
- W2035636775 cites W2028441326 @default.
- W2035636775 cites W2033970010 @default.
- W2035636775 cites W2042703141 @default.
- W2035636775 cites W2047759247 @default.
- W2035636775 cites W2052780078 @default.
- W2035636775 cites W2053350421 @default.
- W2035636775 cites W2054428566 @default.
- W2035636775 cites W2056926456 @default.
- W2035636775 cites W2067826134 @default.
- W2035636775 cites W2069176827 @default.
- W2035636775 cites W2071752035 @default.
- W2035636775 cites W2076345037 @default.
- W2035636775 cites W2077214743 @default.
- W2035636775 cites W2086689509 @default.
- W2035636775 cites W2094547134 @default.
- W2035636775 cites W2104630023 @default.
- W2035636775 cites W2104809172 @default.
- W2035636775 cites W2118937487 @default.
- W2035636775 cites W2121951679 @default.
- W2035636775 cites W2123938765 @default.
- W2035636775 cites W2124440098 @default.
- W2035636775 cites W2132531056 @default.
- W2035636775 cites W2152333152 @default.
- W2035636775 cites W2164618384 @default.
- W2035636775 cites W2165306744 @default.
- W2035636775 cites W4240053492 @default.
- W2035636775 doi "https://doi.org/10.1093/nar/gkg910" @default.
- W2035636775 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/290277" @default.
- W2035636775 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14627820" @default.
- W2035636775 hasPublicationYear "2003" @default.
- W2035636775 type Work @default.
- W2035636775 sameAs 2035636775 @default.
- W2035636775 citedByCount "149" @default.
- W2035636775 countsByYear W20356367752012 @default.
- W2035636775 countsByYear W20356367752013 @default.
- W2035636775 countsByYear W20356367752014 @default.
- W2035636775 countsByYear W20356367752015 @default.
- W2035636775 countsByYear W20356367752016 @default.
- W2035636775 countsByYear W20356367752017 @default.
- W2035636775 countsByYear W20356367752019 @default.
- W2035636775 countsByYear W20356367752020 @default.
- W2035636775 countsByYear W20356367752021 @default.
- W2035636775 countsByYear W20356367752022 @default.
- W2035636775 countsByYear W20356367752023 @default.
- W2035636775 crossrefType "journal-article" @default.
- W2035636775 hasAuthorship W2035636775A5043517944 @default.
- W2035636775 hasAuthorship W2035636775A5052007480 @default.
- W2035636775 hasAuthorship W2035636775A5054222559 @default.
- W2035636775 hasAuthorship W2035636775A5066552100 @default.
- W2035636775 hasBestOaLocation W20356367751 @default.
- W2035636775 hasConcept C104317684 @default.
- W2035636775 hasConcept C12455157 @default.
- W2035636775 hasConcept C133288520 @default.
- W2035636775 hasConcept C138885662 @default.
- W2035636775 hasConcept C141231307 @default.
- W2035636775 hasConcept C157887575 @default.
- W2035636775 hasConcept C160775987 @default.
- W2035636775 hasConcept C172510086 @default.
- W2035636775 hasConcept C2776878037 @default.
- W2035636775 hasConcept C2779535977 @default.
- W2035636775 hasConcept C41895202 @default.
- W2035636775 hasConcept C4918238 @default.
- W2035636775 hasConcept C54355233 @default.
- W2035636775 hasConcept C86803240 @default.
- W2035636775 hasConceptScore W2035636775C104317684 @default.