Matches in SemOpenAlex for { <https://semopenalex.org/work/W2035650904> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2035650904 endingPage "201" @default.
- W2035650904 startingPage "196" @default.
- W2035650904 abstract "In‐gel digestion followed by LC / MS / MS is widely used for the identification of trace amounts of proteins and for the site‐specific glycosylation analysis of glycoproteins in cells and tissues. A major limitation of this technique is the difficulty in acquiring reliable mass spectra for peptides present in minute quantities and glycopeptides with high heterogeneity and poor hydrophobicity. It is considered that the SDS used in electrophoresis can interact with proteins noncovalently and impede the ionization of peptides/glycopeptides. In this study, we report an improved in‐gel digestion method to acquire reliable mass spectra of a trace amount of peptides/glycopeptides. A key innovation of our improved method is the use of guanidine hydrochloride, which forms complexes with the residual SDS molecules in the sample. The precipitation and removal of SDS by addition of the guanidine hydrochloride was successful in improving the S / N of peptides/glycopeptides in mass spectra and acquiring a more comprehensive MS / MS data set for the various glycoforms of each glycopeptide." @default.
- W2035650904 created "2016-06-24" @default.
- W2035650904 creator A5037222124 @default.
- W2035650904 creator A5084056414 @default.
- W2035650904 creator A5084797764 @default.
- W2035650904 date "2014-01-25" @default.
- W2035650904 modified "2023-10-16" @default.
- W2035650904 title "An improved in-gel digestion method for efficient identification of protein and glycosylation analysis of glycoproteins using guanidine hydrochloride" @default.
- W2035650904 cites W1523851360 @default.
- W2035650904 cites W163526048 @default.
- W2035650904 cites W1975304761 @default.
- W2035650904 cites W2004125187 @default.
- W2035650904 cites W2005305877 @default.
- W2035650904 cites W2024191848 @default.
- W2035650904 cites W2100837269 @default.
- W2035650904 doi "https://doi.org/10.1002/pmic.201300332" @default.
- W2035650904 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24272977" @default.
- W2035650904 hasPublicationYear "2014" @default.
- W2035650904 type Work @default.
- W2035650904 sameAs 2035650904 @default.
- W2035650904 citedByCount "15" @default.
- W2035650904 countsByYear W20356509042015 @default.
- W2035650904 countsByYear W20356509042016 @default.
- W2035650904 countsByYear W20356509042017 @default.
- W2035650904 countsByYear W20356509042018 @default.
- W2035650904 countsByYear W20356509042019 @default.
- W2035650904 countsByYear W20356509042020 @default.
- W2035650904 countsByYear W20356509042021 @default.
- W2035650904 crossrefType "journal-article" @default.
- W2035650904 hasAuthorship W2035650904A5037222124 @default.
- W2035650904 hasAuthorship W2035650904A5084056414 @default.
- W2035650904 hasAuthorship W2035650904A5084797764 @default.
- W2035650904 hasConcept C108625454 @default.
- W2035650904 hasConcept C137610392 @default.
- W2035650904 hasConcept C162356407 @default.
- W2035650904 hasConcept C185592680 @default.
- W2035650904 hasConcept C2776460385 @default.
- W2035650904 hasConcept C2777313579 @default.
- W2035650904 hasConcept C2779281246 @default.
- W2035650904 hasConcept C2779463914 @default.
- W2035650904 hasConcept C2781357212 @default.
- W2035650904 hasConcept C43617362 @default.
- W2035650904 hasConcept C501593827 @default.
- W2035650904 hasConcept C55493867 @default.
- W2035650904 hasConceptScore W2035650904C108625454 @default.
- W2035650904 hasConceptScore W2035650904C137610392 @default.
- W2035650904 hasConceptScore W2035650904C162356407 @default.
- W2035650904 hasConceptScore W2035650904C185592680 @default.
- W2035650904 hasConceptScore W2035650904C2776460385 @default.
- W2035650904 hasConceptScore W2035650904C2777313579 @default.
- W2035650904 hasConceptScore W2035650904C2779281246 @default.
- W2035650904 hasConceptScore W2035650904C2779463914 @default.
- W2035650904 hasConceptScore W2035650904C2781357212 @default.
- W2035650904 hasConceptScore W2035650904C43617362 @default.
- W2035650904 hasConceptScore W2035650904C501593827 @default.
- W2035650904 hasConceptScore W2035650904C55493867 @default.
- W2035650904 hasFunder F4320320912 @default.
- W2035650904 hasFunder F4320324144 @default.
- W2035650904 hasIssue "2-3" @default.
- W2035650904 hasLocation W20356509041 @default.
- W2035650904 hasLocation W20356509042 @default.
- W2035650904 hasOpenAccess W2035650904 @default.
- W2035650904 hasPrimaryLocation W20356509041 @default.
- W2035650904 hasRelatedWork W1968684223 @default.
- W2035650904 hasRelatedWork W1990659752 @default.
- W2035650904 hasRelatedWork W2009099364 @default.
- W2035650904 hasRelatedWork W2025873169 @default.
- W2035650904 hasRelatedWork W2090475715 @default.
- W2035650904 hasRelatedWork W2139575802 @default.
- W2035650904 hasRelatedWork W2262250366 @default.
- W2035650904 hasRelatedWork W2354066328 @default.
- W2035650904 hasRelatedWork W3148068717 @default.
- W2035650904 hasRelatedWork W2188816439 @default.
- W2035650904 hasVolume "14" @default.
- W2035650904 isParatext "false" @default.
- W2035650904 isRetracted "false" @default.
- W2035650904 magId "2035650904" @default.
- W2035650904 workType "article" @default.