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• W2035672458 abstract "An electrochemical immunosensor was constructed using an electropolymerized pyrrolepropylic acid (PPA) film with high porosity and hydrophilicity. A high density of carboxyl groups of PPA was used to covalently attach protein probes, leading to significantly improved detection sensitivity compared with conventional entrapment methods. As a model, anti-mouse IgG was covalently immobilized or entrapped in the PPA film and used in a sandwich-type alkaline phosphatase-catalyzing amperometric immunoassay with p-aminophenyl phosphate as the substrate. With covalent binding, the detection limit for IgG in PBS buffer, pH 7.4, was 100 pg/mL with a dynamic range of 5 orders of magnitude. The covalent bonding mode in the carbonate−bicarbonate buffer, pH 9.6, further brought down the detection limit to 20 pg/mL with remarkable selectivity." @default.
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• W2035672458 date "2006-09-22" @default.
• W2035672458 modified "2023-10-15" @default.
• W2035672458 title "Sensitive Amperometric Immunosensing Using Polypyrrolepropylic Acid Films for Biomolecule Immobilization" @default.
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• W2035672458 doi "https://doi.org/10.1021/ac060657o" @default.
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