FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2035684482> ?p ?o ?g. }

• W2035684482 abstract "The current prognosis and classification of CRC relies on staging systems that integrate histopathologic and clinical findings. However, in the majority of CRC cases, cell dysfunction is the result of numerous mutations that modify protein expression and post-translational modification(1). A number of cell surface antigens, including cluster of differentiation (CD) antigens, have been identified as potential prognostic or metastatic biomarkers in CRC. These antigens make ideal biomarkers as their expression often changes with tumour progression or interactions with other cell types, such as tumour-infiltrating lymphocytes (TILs) and tumour-associated macrophages (TAMs). The use of immunohistochemistry (IHC) for cancer sub-classification and prognostication is well established for some tumour types(2,3). However, no single 'marker' has shown prognostic significance greater than clinico-pathological staging or gained wide acceptance for use in routine pathology reporting of all CRC cases. A more recent approach to prognostic stratification of disease phenotypes relies on surface protein profiles using multiple 'markers'. While expression profiling of tumours using proteomic techniques such as iTRAQ is a powerful tool for the discovery of biomarkers4, it is not optimal for routine use in diagnostic laboratories and cannot distinguish different cell types in a mixed population. In addition, large amounts of tumour tissue are required for the profiling of purified plasma membrane glycoproteins by these methods. In this video we described a simple method for surface proteome profiling of viable cells from disaggregated CRC samples using a DotScan CRC antibody microarray. The 122-antibody microarray consists of a standard 82-antibody region recognizing a range of lineage-specific leukocyte markers, adhesion molecules, receptors and markers of inflammation and immune response(5), together with a satellite region for detection of 40 potentially prognostic markers for CRC. Cells are captured only on antibodies for which they express the corresponding antigen. The cell density per dot, determined by optical scanning, reflects the proportion of cells expressing that antigen, the level of expression of the antigen and affinity of the antibody(6). For CRC tissue or normal intestinal mucosa, optical scans reflect the immunophenotype of mixed populations of cells. Fluorescence multiplexing can then be used to profile selected sub-populations of cells of interest captured on the array. For example, Alexa 647-anti-epithelial cell adhesion molecule (EpCAM; CD326), is a pan-epithelial differentiation antigen that was used to detect CRC cells and also epithelial cells of normal intestinal mucosa, while Phycoerythrin-anti-CD3, was used to detect infiltrating T-cells(7). The DotScan CRC microarray should be the prototype for a diagnostic alternative to the anatomically-based CRC staging system." @default.
• W2035684482 created "2016-06-24" @default.
• W2035684482 creator A5016929277 @default.
• W2035684482 creator A5027566677 @default.
• W2035684482 creator A5060274786 @default.
• W2035684482 creator A5063111253 @default.
• W2035684482 creator A5072327609 @default.
• W2035684482 creator A5079906205 @default.
• W2035684482 date "2011-09-25" @default.
• W2035684482 modified "2023-09-23" @default.
• W2035684482 title "Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing" @default.
• W2035684482 cites W1605230984 @default.
• W2035684482 cites W1788506364 @default.
• W2035684482 cites W196157878 @default.
• W2035684482 cites W1983851456 @default.
• W2035684482 cites W2020960488 @default.
• W2035684482 cites W2061429719 @default.
• W2035684482 cites W2100668965 @default.
• W2035684482 cites W2169560327 @default.
• W2035684482 cites W2312288626 @default.
• W2035684482 cites W2399597901 @default.
• W2035684482 cites W45534775 @default.
• W2035684482 doi "https://doi.org/10.3791/3322" @default.
• W2035684482 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3230216" @default.
• W2035684482 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21968569" @default.
• W2035684482 hasPublicationYear "2011" @default.
• W2035684482 type Work @default.
• W2035684482 sameAs 2035684482 @default.
• W2035684482 citedByCount "12" @default.
• W2035684482 countsByYear W20356844822012 @default.
• W2035684482 countsByYear W20356844822013 @default.
• W2035684482 countsByYear W20356844822014 @default.
• W2035684482 countsByYear W20356844822015 @default.
• W2035684482 countsByYear W20356844822016 @default.
• W2035684482 countsByYear W20356844822017 @default.
• W2035684482 countsByYear W20356844822019 @default.
• W2035684482 countsByYear W20356844822020 @default.
• W2035684482 countsByYear W20356844822021 @default.
• W2035684482 crossrefType "journal-article" @default.
• W2035684482 hasAuthorship W2035684482A5016929277 @default.
• W2035684482 hasAuthorship W2035684482A5027566677 @default.
• W2035684482 hasAuthorship W2035684482A5060274786 @default.
• W2035684482 hasAuthorship W2035684482A5063111253 @default.
• W2035684482 hasAuthorship W2035684482A5072327609 @default.
• W2035684482 hasAuthorship W2035684482A5079906205 @default.
• W2035684482 hasBestOaLocation W20356844822 @default.
• W2035684482 hasConcept C104317684 @default.
• W2035684482 hasConcept C121608353 @default.
• W2035684482 hasConcept C126322002 @default.
• W2035684482 hasConcept C127716648 @default.
• W2035684482 hasConcept C142724271 @default.
• W2035684482 hasConcept C147483822 @default.
• W2035684482 hasConcept C1491633281 @default.
• W2035684482 hasConcept C150194340 @default.
• W2035684482 hasConcept C159654299 @default.
• W2035684482 hasConcept C171196145 @default.
• W2035684482 hasConcept C18431079 @default.
• W2035684482 hasConcept C186836561 @default.
• W2035684482 hasConcept C193270364 @default.
• W2035684482 hasConcept C195814341 @default.
• W2035684482 hasConcept C203014093 @default.
• W2035684482 hasConcept C204232928 @default.
• W2035684482 hasConcept C2908647359 @default.
• W2035684482 hasConcept C40722700 @default.
• W2035684482 hasConcept C502942594 @default.
• W2035684482 hasConcept C526805850 @default.
• W2035684482 hasConcept C54355233 @default.
• W2035684482 hasConcept C55493867 @default.
• W2035684482 hasConcept C71924100 @default.
• W2035684482 hasConcept C86803240 @default.
• W2035684482 hasConcept C99454951 @default.
• W2035684482 hasConceptScore W2035684482C104317684 @default.
• W2035684482 hasConceptScore W2035684482C121608353 @default.
• W2035684482 hasConceptScore W2035684482C126322002 @default.
• W2035684482 hasConceptScore W2035684482C127716648 @default.
• W2035684482 hasConceptScore W2035684482C142724271 @default.
• W2035684482 hasConceptScore W2035684482C147483822 @default.
• W2035684482 hasConceptScore W2035684482C1491633281 @default.
• W2035684482 hasConceptScore W2035684482C150194340 @default.
• W2035684482 hasConceptScore W2035684482C159654299 @default.
• W2035684482 hasConceptScore W2035684482C171196145 @default.
• W2035684482 hasConceptScore W2035684482C18431079 @default.
• W2035684482 hasConceptScore W2035684482C186836561 @default.
• W2035684482 hasConceptScore W2035684482C193270364 @default.
• W2035684482 hasConceptScore W2035684482C195814341 @default.
• W2035684482 hasConceptScore W2035684482C203014093 @default.
• W2035684482 hasConceptScore W2035684482C204232928 @default.
• W2035684482 hasConceptScore W2035684482C2908647359 @default.
• W2035684482 hasConceptScore W2035684482C40722700 @default.
• W2035684482 hasConceptScore W2035684482C502942594 @default.
• W2035684482 hasConceptScore W2035684482C526805850 @default.
• W2035684482 hasConceptScore W2035684482C54355233 @default.
• W2035684482 hasConceptScore W2035684482C55493867 @default.
• W2035684482 hasConceptScore W2035684482C71924100 @default.
• W2035684482 hasConceptScore W2035684482C86803240 @default.
• W2035684482 hasConceptScore W2035684482C99454951 @default.
• W2035684482 hasIssue "55" @default.
• W2035684482 hasLocation W20356844821 @default.
• W2035684482 hasLocation W20356844822 @default.
• W2035684482 hasLocation W20356844823 @default.

• next