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- W2035704887 abstract "High-resolution thermal denaturation was used to measure the heterogeneity within repeated DNA sequences. An analysis of combined denaturation/redenaturation experiments on mouse satellite DNA suggests the existence of two minor components, one of which does not appear in the prepared EcoRII monomer. The resolving power of the denaturation/redenaturation experiment is estimated and contrasted with that of the reassociation experiment, often used to estimate repeated sequence heterogeneity. A mathematical model of the redenaturation experiment was developed and applied to mouse satellite data; the results suggest that only one-fourth of the mismatched base pairs are energetically significant in the reduction of heteroduplex stability." @default.
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- W2035704887 date "1984-03-01" @default.
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- W2035704887 title "An analysis of repeated sequence heterogeneity" @default.
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- W2035704887 doi "https://doi.org/10.1016/0003-9861(84)90181-4" @default.
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