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- W2035722210 abstract "Strains of the bacterium, Pseudomonas sp., causing blight of kiwifruit, isolated from affected vines in New Zealand and in the People's Republic of China, were compared using DNA-DNA hybridization and repetitive-PCR (rep-PCR) and biolog. DNA of each strain was hybridized to DNA of reference strains representing P. viridiflava, P. savastanoi and the kiwifruit pathogen. Strains of the pathogen from New Zealand and China shared reassociation values exceeding 76%. Strains from both countries shared reassociation values of more than 66% (average 82%) with DNA of the type strain of P. savastanoi, and less than 51% with DNA of P. viridiflava (average 33.5%). A comparison by rep-PCR using the primers REP, ERIC and BOX showed that strains from New Zealand and China were indistinguishable. Comparison of endonuclease restriction patterns of strains from New Zealand showed that those gathered over a 20-year period from different growing regions were more diverse than strains gathered in one orchard in a single season. The ecological implications of these data and the source of the pathogen in New Zealand are discussed. It is concluded that the pathogen originated in China and was introduced into New Zealand in vegetative kiwifruit material, and not as was previously thought, in two small seed samples.ZusammenfassungBelege dafur, das der durch Pseudomonas sp. verursachte Bakterienbrand der Kiwi aus China nach Neuseeland eingeschleppt wurdeStamme des Bakteriums Pseudomonas sp., die einen Bakterienbrand der Kiwi hervorrufen, wurden aus befallenen Pflanzen in Neuseeland und in der Volksrepublic China isoliert und mit Hilfe der DNA-DNA-Hybridisierung und repetitiver PCR (rep-PCR) verglichen. DNA jedes Stamms wurde an DNA von Referenzstammen hybridisiert, welche Pseudomonas viridiflava, P. savanastoi und das Pathogen der Kiwi reprasentierten. Die neuseelandischen und die chinesischen Stamme des Pathogens zeigten Reassoziierungswerte von mehr als 76%. Die Stamme aus beiden Landern zeigten Reassoziierungswerte von mehr als 66% (Durchschnitt 82%) mit DNA des Typstamms P. savanastoi. und weniger als 51% mit DNA von P. viridiflava (Durschschnitt 33,5%). Ein Vergleich mit rep-PCR unter Verwendung der Primer REP, ERIC und BOX zeigte, das die Stamme aus Neuseeland und China nicht voneinander zu unterscheiden waren. Ein Vergleich der Endonuclease-Restriktionsmuster der neuseelandischen Stamme ergab, das Stamme, die uber einen Zeitraum von 20 Jahren in unterschiedlichen Anbaugebieten gesammelt worden waren, mehr Diversitat zeigten als Stamme, die wahrend einer Vegetationsperiode in nur einer Anlage gesammelt worden waren. Die okologische Bedeutung dieser Daten und die Herkunft des Pathogens in Neuseeland werden diskutiert. Es wird gefolgert, das das Pathogen aus China stammt und in vegetativem Material der Kiwipflanze nach Neuseeland gelangte - nicht, wie zuvor angenom-men wurde, in zwei kleinen Samenproben." @default.
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- W2035722210 date "1999-02-01" @default.
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- W2035722210 title "Evidence that Bacterial Blight of Kiwifruit, Caused by a Pseudomonas sp., was Introduced into New Zealand From China" @default.
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- W2035722210 doi "https://doi.org/10.1046/j.1439-0434.1999.147002089.x" @default.
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