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- W2035736181 abstract "1. Tissue slices from Morris hepatoma 7777 were incubated for 3 hr in Krebs-Ringer-bicarbonate solution and the cell-free supernatants were used for isolating alpha-fetoprotein. 2. Purification procedure included salting out with ammonium sulphate, affinity chromatography (either Concanavalin A-Sepharose or Cibacron-Blue-Sepharose), hydrophobic chromatography and preparative polyacrylamide gel electrophoresis (PAGE). 3.alpha-Fetoprotein was obtained with a yield of approx. 10% and in pure form, as judged from crossed immunoelectrophoresis and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)." @default.
- W2035736181 created "2016-06-24" @default.
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- W2035736181 date "1982-01-01" @default.
- W2035736181 modified "2023-09-27" @default.
- W2035736181 title "ISolation of rat α-fetoprotein from morris hepatoma 7777" @default.
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- W2035736181 doi "https://doi.org/10.1016/0020-711x(82)90049-0" @default.
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