Matches in SemOpenAlex for { <https://semopenalex.org/work/W2035787963> ?p ?o ?g. }
- W2035787963 endingPage "531" @default.
- W2035787963 startingPage "526" @default.
- W2035787963 abstract "The effect of human recombinant IL-1 receptor antagonist (hrIL-1Ra) on leukotriene B4 (LTB4) release was investigated in activated human monocyte cultures. To stimulate LTB4 generation, LPS was used as an agonist. Detection was performed with the highly sensitive radioimmunoassay method. The cells were treated with scalar concentrations using LPS at 1-1000 ng/ml for different periods of time. The greater LTB4 stimulation was found at LPS 100 ng/ml for 18 h incubation time. Preincubation of monocytes with cytochalasin B (CB) (5 micrograms/ml) for 15 min augmented the release of LTB4 when LPS was used. A dose-dependent inhibition was found when human monocytes were pretreated for 10 min with hrIL-1Ra at different concentrations (0.25-250 ng/ml) and then treated with LPS 100 ng/ml for 18 h. Maximum inhibition was observed at the highest concentration of hrIL-1Ra (250 ng/ml). Macrophages treated with a non-selective 5-lipoxygenase inhibitor, nordihydroguaiaretic acid (NDGA), used at 10 microM, added 15 min before LPS 100 ng/ml, produce a dose-dependent inhibition of LTB4. Cells pretreated with arachidonic acid, at various concentrations (10(-9)-10(-5) M) for 10 min and then treated with LPS 100 ng/ml for 18 h, were also inhibited in a dose-dependent manner by hrIL-1Ra in their production of LTB4. The inhibition of LTB4 release by hrIL-1Ra, in LPS-stimulated human monocytes, may suggest an important modulatory role for this new cytokine (monokine) in inflammation and immunity and may hold future therapeutic implications for diseases involving LTB4 as a mediator." @default.
- W2035787963 created "2016-06-24" @default.
- W2035787963 creator A5001309433 @default.
- W2035787963 creator A5009618806 @default.
- W2035787963 creator A5016103963 @default.
- W2035787963 creator A5037842162 @default.
- W2035787963 creator A5052797072 @default.
- W2035787963 creator A5054106836 @default.
- W2035787963 date "1993-03-01" @default.
- W2035787963 modified "2023-09-25" @default.
- W2035787963 title "Human recombinant IL-1 receptor antagonist (IL-IRa) inhibits leukotriene B4 generation from human monocyte suspensions stimulated by lipopolysaccharide (LPS)" @default.
- W2035787963 cites W1480708545 @default.
- W2035787963 cites W1522651144 @default.
- W2035787963 cites W1523934634 @default.
- W2035787963 cites W1544236481 @default.
- W2035787963 cites W1574330564 @default.
- W2035787963 cites W1864533597 @default.
- W2035787963 cites W1971402663 @default.
- W2035787963 cites W1973576012 @default.
- W2035787963 cites W1973832079 @default.
- W2035787963 cites W1978411769 @default.
- W2035787963 cites W1978580827 @default.
- W2035787963 cites W1989459539 @default.
- W2035787963 cites W2007210793 @default.
- W2035787963 cites W2023213942 @default.
- W2035787963 cites W2025001062 @default.
- W2035787963 cites W2066542248 @default.
- W2035787963 cites W2071794877 @default.
- W2035787963 cites W2073598149 @default.
- W2035787963 cites W2073977304 @default.
- W2035787963 cites W2074393911 @default.
- W2035787963 cites W2076658850 @default.
- W2035787963 cites W2077062138 @default.
- W2035787963 cites W2081462803 @default.
- W2035787963 cites W2083611460 @default.
- W2035787963 cites W2089356284 @default.
- W2035787963 cites W2092515987 @default.
- W2035787963 cites W2093565920 @default.
- W2035787963 cites W2120179212 @default.
- W2035787963 cites W2135745523 @default.
- W2035787963 cites W2160782061 @default.
- W2035787963 cites W2166542918 @default.
- W2035787963 cites W2404070385 @default.
- W2035787963 cites W2441730478 @default.
- W2035787963 cites W3025329443 @default.
- W2035787963 cites W4229688240 @default.
- W2035787963 cites W4232576781 @default.
- W2035787963 cites W87082738 @default.
- W2035787963 cites W2031033330 @default.
- W2035787963 doi "https://doi.org/10.1111/j.1365-2249.1993.tb05936.x" @default.
- W2035787963 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1554727" @default.
- W2035787963 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8383025" @default.
- W2035787963 hasPublicationYear "1993" @default.
- W2035787963 type Work @default.
- W2035787963 sameAs 2035787963 @default.
- W2035787963 citedByCount "17" @default.
- W2035787963 countsByYear W20357879632015 @default.
- W2035787963 countsByYear W20357879632016 @default.
- W2035787963 countsByYear W20357879632021 @default.
- W2035787963 countsByYear W20357879632022 @default.
- W2035787963 crossrefType "journal-article" @default.
- W2035787963 hasAuthorship W2035787963A5001309433 @default.
- W2035787963 hasAuthorship W2035787963A5009618806 @default.
- W2035787963 hasAuthorship W2035787963A5016103963 @default.
- W2035787963 hasAuthorship W2035787963A5037842162 @default.
- W2035787963 hasAuthorship W2035787963A5052797072 @default.
- W2035787963 hasAuthorship W2035787963A5054106836 @default.
- W2035787963 hasBestOaLocation W20357879631 @default.
- W2035787963 hasConcept C126322002 @default.
- W2035787963 hasConcept C134018914 @default.
- W2035787963 hasConcept C170493617 @default.
- W2035787963 hasConcept C181199279 @default.
- W2035787963 hasConcept C185592680 @default.
- W2035787963 hasConcept C202751555 @default.
- W2035787963 hasConcept C24998067 @default.
- W2035787963 hasConcept C2776042228 @default.
- W2035787963 hasConcept C2776452501 @default.
- W2035787963 hasConcept C2776685179 @default.
- W2035787963 hasConcept C2776885963 @default.
- W2035787963 hasConcept C2776914184 @default.
- W2035787963 hasConcept C2778078955 @default.
- W2035787963 hasConcept C2778122271 @default.
- W2035787963 hasConcept C2778690821 @default.
- W2035787963 hasConcept C2778754761 @default.
- W2035787963 hasConcept C2778870864 @default.
- W2035787963 hasConcept C2778938600 @default.
- W2035787963 hasConcept C2779603739 @default.
- W2035787963 hasConcept C2781184567 @default.
- W2035787963 hasConcept C55493867 @default.
- W2035787963 hasConcept C71924100 @default.
- W2035787963 hasConcept C74172505 @default.
- W2035787963 hasConcept C98274493 @default.
- W2035787963 hasConceptScore W2035787963C126322002 @default.
- W2035787963 hasConceptScore W2035787963C134018914 @default.
- W2035787963 hasConceptScore W2035787963C170493617 @default.
- W2035787963 hasConceptScore W2035787963C181199279 @default.
- W2035787963 hasConceptScore W2035787963C185592680 @default.
- W2035787963 hasConceptScore W2035787963C202751555 @default.