FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2035849984> ?p ?o ?g. }

• W2035849984 endingPage "9953" @default.
• W2035849984 startingPage "9945" @default.
• W2035849984 abstract "Despite the recent advances in structural analysis of monoclonal antibodies with bottom-up, middle-down, and top-down mass spectrometry (MS), further improvements in analysis accuracy, depth, and speed are needed. The remaining challenges include quantitatively accurate assignment of post-translational modifications, reduction of artifacts introduced during sample preparation, increased sequence coverage per liquid chromatography (LC) MS experiment, and ability to extend the detailed characterization to simple antibody cocktails and more complex antibody mixtures. Here, we evaluate the recently introduced extended bottom-up proteomics (eBUP) approach based on proteolysis with secreted aspartic protease 9, Sap9, for analysis of monoclonal antibodies. Key findings of the Sap9-based proteomics analysis of a single antibody include: (i) extensive antibody sequence coverage with up to 100% for the light chain and up to 99-100% for the heavy chain in a single LC-MS run; (ii) connectivity of complementarity-determining regions (CDRs) via Sap9-produced large proteolytic peptides (3.4 kDa on average) containing up to two CDRs per peptide; (iii) reduced artifact introduction (e. g., deamidation) during proteolysis with Sap9 compared to conventional bottom-up proteomics workflows. The analysis of a mixture of six antibodies via Sap9-based eBUP produced comparable results. Due to the reasons specified above, Sap9-produced proteolytic peptides improve the identification confidence of antibodies from the mixtures compared to conventional bottom-up proteomics dealing with shorter proteolytic peptides." @default.
• W2035849984 created "2016-06-24" @default.
• W2035849984 creator A5004595287 @default.
• W2035849984 creator A5022292935 @default.
• W2035849984 creator A5031691939 @default.
• W2035849984 creator A5035195284 @default.
• W2035849984 creator A5044551354 @default.
• W2035849984 creator A5075723932 @default.
• W2035849984 creator A5077227657 @default.
• W2035849984 date "2014-09-24" @default.
• W2035849984 modified "2023-10-03" @default.
• W2035849984 title "Advantages of Extended Bottom-Up Proteomics Using Sap9 for Analysis of Monoclonal Antibodies" @default.
• W2035849984 cites W1550340517 @default.
• W2035849984 cites W1967681366 @default.
• W2035849984 cites W1969050666 @default.
• W2035849984 cites W1972249283 @default.
• W2035849984 cites W1972913960 @default.
• W2035849984 cites W1975086142 @default.
• W2035849984 cites W1979626824 @default.
• W2035849984 cites W2005088298 @default.
• W2035849984 cites W2007167393 @default.
• W2035849984 cites W2007601531 @default.
• W2035849984 cites W2008863376 @default.
• W2035849984 cites W2016938382 @default.
• W2035849984 cites W2029325590 @default.
• W2035849984 cites W2033701042 @default.
• W2035849984 cites W2034465573 @default.
• W2035849984 cites W2045877726 @default.
• W2035849984 cites W2051764215 @default.
• W2035849984 cites W2056580789 @default.
• W2035849984 cites W2059845531 @default.
• W2035849984 cites W2064868949 @default.
• W2035849984 cites W2073176892 @default.
• W2035849984 cites W2074975150 @default.
• W2035849984 cites W2078594804 @default.
• W2035849984 cites W2104943097 @default.
• W2035849984 cites W2106860332 @default.
• W2035849984 cites W2121612064 @default.
• W2035849984 cites W2131741443 @default.
• W2035849984 cites W2167056206 @default.
• W2035849984 cites W2169811127 @default.
• W2035849984 doi "https://doi.org/10.1021/ac502766n" @default.
• W2035849984 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25207962" @default.
• W2035849984 hasPublicationYear "2014" @default.
• W2035849984 type Work @default.
• W2035849984 sameAs 2035849984 @default.
• W2035849984 citedByCount "47" @default.
• W2035849984 countsByYear W20358499842015 @default.
• W2035849984 countsByYear W20358499842016 @default.
• W2035849984 countsByYear W20358499842017 @default.
• W2035849984 countsByYear W20358499842018 @default.
• W2035849984 countsByYear W20358499842019 @default.
• W2035849984 countsByYear W20358499842020 @default.
• W2035849984 countsByYear W20358499842021 @default.
• W2035849984 countsByYear W20358499842022 @default.
• W2035849984 countsByYear W20358499842023 @default.
• W2035849984 crossrefType "journal-article" @default.
• W2035849984 hasAuthorship W2035849984A5004595287 @default.
• W2035849984 hasAuthorship W2035849984A5022292935 @default.
• W2035849984 hasAuthorship W2035849984A5031691939 @default.
• W2035849984 hasAuthorship W2035849984A5035195284 @default.
• W2035849984 hasAuthorship W2035849984A5044551354 @default.
• W2035849984 hasAuthorship W2035849984A5075723932 @default.
• W2035849984 hasAuthorship W2035849984A5077227657 @default.
• W2035849984 hasConcept C104317684 @default.
• W2035849984 hasConcept C112979427 @default.
• W2035849984 hasConcept C159654299 @default.
• W2035849984 hasConcept C162356407 @default.
• W2035849984 hasConcept C181199279 @default.
• W2035849984 hasConcept C185592680 @default.
• W2035849984 hasConcept C202157981 @default.
• W2035849984 hasConcept C203014093 @default.
• W2035849984 hasConcept C2780784506 @default.
• W2035849984 hasConcept C2781307694 @default.
• W2035849984 hasConcept C31827203 @default.
• W2035849984 hasConcept C36394416 @default.
• W2035849984 hasConcept C43617362 @default.
• W2035849984 hasConcept C46111723 @default.
• W2035849984 hasConcept C542903549 @default.
• W2035849984 hasConcept C55493867 @default.
• W2035849984 hasConcept C70721500 @default.
• W2035849984 hasConcept C86803240 @default.
• W2035849984 hasConceptScore W2035849984C104317684 @default.
• W2035849984 hasConceptScore W2035849984C112979427 @default.
• W2035849984 hasConceptScore W2035849984C159654299 @default.
• W2035849984 hasConceptScore W2035849984C162356407 @default.
• W2035849984 hasConceptScore W2035849984C181199279 @default.
• W2035849984 hasConceptScore W2035849984C185592680 @default.
• W2035849984 hasConceptScore W2035849984C202157981 @default.
• W2035849984 hasConceptScore W2035849984C203014093 @default.
• W2035849984 hasConceptScore W2035849984C2780784506 @default.
• W2035849984 hasConceptScore W2035849984C2781307694 @default.
• W2035849984 hasConceptScore W2035849984C31827203 @default.
• W2035849984 hasConceptScore W2035849984C36394416 @default.
• W2035849984 hasConceptScore W2035849984C43617362 @default.
• W2035849984 hasConceptScore W2035849984C46111723 @default.
• W2035849984 hasConceptScore W2035849984C542903549 @default.
• W2035849984 hasConceptScore W2035849984C55493867 @default.

• next