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• W2035854223 startingPage "474" @default.
• W2035854223 abstract "In a typical preparation of aquomethemoglobin, oxyhemoglobin is oxidized with potassium ferricyanide, and the resultant mixture of methemoglobin and potassium ferro- and ferricyanides is separated on a Sephadex G-25 column. We find that about 1% of the heme is reduced on the column and is eluted with the methemoglobin. Magnetic circular dichroism spectra show that the reduced species is oxyhemoglobin. Magnetic circular dichroism is more sensitive than is absorption spectroscopy to small amounts of oxyhemoglobin in such solutions; we can detect its presence at the 0.1% level. A redetermination of the extinction coefficients for methemoglobin gives a value of 0.80 for the absorbance ratio A570A630 at pH 6. This value lies clearly outside the currently accepted range of 0.83 to 0.87." @default.
• W2035854223 created "2016-06-24" @default.
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• W2035854223 date "1978-10-01" @default.
• W2035854223 modified "2023-09-26" @default.
• W2035854223 title "Partial reduction of aquomethemoglobin on a sephadex G-25 column as detected by magnetic circular dichroism spectroscopy and revised extinction coefficients for aquomethemoglobin" @default.
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• W2035854223 doi "https://doi.org/10.1016/0003-2697(78)90140-9" @default.
• W2035854223 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/727492" @default.
• W2035854223 hasPublicationYear "1978" @default.
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