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• W2035871736 endingPage "2649" @default.
• W2035871736 startingPage "2638" @default.
• W2035871736 abstract "The influence of cholesterol and POPE on lung surfactant model systems consisting of DPPC/DPPG (80:20) and DPPC/DPPG/surfactant protein C (80:20:0.4) has been investigated. Cholesterol leads to a condensation of the monolayers, whereas the isotherms of model lung surfactant films containing POPE exhibit a slight expansion combined with an increased compressibility at medium surface pressure (10–30 mN/m). An increasing amount of liquid-expanded domains can be visualized by means of fluorescence light microscopy in lung surfactant monolayers after addition of either cholesterol or POPE. At surface pressures of 50 mN/m, protrusions are formed which differ in size and shape as a function of the content of cholesterol or POPE, but only if SP-C is present. Low amounts of cholesterol (10 mol %) lead to an increasing number of protrusions, which also grow in size. This is interpreted as a stabilizing effect of cholesterol on bilayers formed underneath the monolayer. Extreme amounts of cholesterol (30 mol %), however, cause an increased monolayer rigidity, thus preventing reversible multilayer formation. In contrast, POPE, as a nonbilayer lipid thought to stabilize the edges of protrusions, leads to more narrow protrusions. The lateral extension of the protrusions is thereby more influenced than their height." @default.
• W2035871736 created "2016-06-24" @default.
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• W2035871736 date "2005-04-01" @default.
• W2035871736 modified "2023-10-18" @default.
• W2035871736 title "Multilayer Structures in Lipid Monolayer Films Containing Surfactant Protein C: Effects of Cholesterol and POPE" @default.
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• W2035871736 doi "https://doi.org/10.1529/biophysj.104.050823" @default.
• W2035871736 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1305360" @default.
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