FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2035943929> ?p ?o ?g. }

• W2035943929 endingPage "1316" @default.
• W2035943929 startingPage "1308" @default.
• W2035943929 abstract "Singlet oxygen (1O2) is a reactive oxygen species generated during photo-oxidation, inflammation, and via peroxidase-catalyzed reactions (e.g., myeloperoxidase and eosinophil peroxidase). 1O2 oxidizes the free amino acids Trp, Tyr, His, Cys, and Met, and those species present on peptides/proteins, with this resulting in modulation of protein structure and function. Impairment of the activity of antioxidant enzymes may be of relevance to the oxidative stress observed in a number of pathologies involving either light exposure or inflammation. In this study, the effects of 1O2 on glutathione peroxidase (GPx) and thioredoxin reductase (TrxR) activity, including the mechanisms of their inactivation, were investigated. Exposure of GPx or TrxR, either as purified proteins or in cell lysates, to Rose Bengal and visible light (an established source of 1O2) resulted in significant, photolysis time-dependent reductions in enzyme activity (10–40%, P<0.05). More extensive inhibition (ca. 2-fold) was detected when the reactions were carried out in D2O, consistent with the intermediacy of 1O2. No additional inhibition was detected after the cessation of photolysis, eliminating a role for photo-products. Methionine, which reacts rapidly with 1O2 (k∼107 M−1 s−1), significantly reduced photo-inactivation at large molar excesses, presumably by acting as an alternative target. Reductants (NaBH4, DTT, GSH, or NADPH) added after the cessation of 1O2 formation were unable to reverse enzyme inactivation, consistent with irreversible enzyme oxidation. Formation of nonreducible protein aggregates and/or fragments was detected for both photo-oxidized GPx and TrxR by SDS-PAGE. An oxidant concentration-dependent increase in protein carbonyls was detected with TrxR but not GPx. These studies thus demonstrate that the antioxidant enzymes GPx and TrxR can be irreversibly inactivated by 1O2." @default.
• W2035943929 created "2016-06-24" @default.
• W2035943929 creator A5025701621 @default.
• W2035943929 creator A5029985039 @default.
• W2035943929 creator A5069317976 @default.
• W2035943929 date "2012-09-01" @default.
• W2035943929 modified "2023-10-09" @default.
• W2035943929 title "Photo-oxidation-induced inactivation of the selenium-containing protective enzymes thioredoxin reductase and glutathione peroxidase" @default.
• W2035943929 cites W1593876540 @default.
• W2035943929 cites W1963668163 @default.
• W2035943929 cites W1964893456 @default.
• W2035943929 cites W1966838825 @default.
• W2035943929 cites W1972160960 @default.
• W2035943929 cites W1973595325 @default.
• W2035943929 cites W1982333316 @default.
• W2035943929 cites W1986562862 @default.
• W2035943929 cites W1986669241 @default.
• W2035943929 cites W1988760590 @default.
• W2035943929 cites W1988765421 @default.
• W2035943929 cites W1989261646 @default.
• W2035943929 cites W1999842345 @default.
• W2035943929 cites W2000379709 @default.
• W2035943929 cites W2001042048 @default.
• W2035943929 cites W2005752954 @default.
• W2035943929 cites W2010809070 @default.
• W2035943929 cites W2013356870 @default.
• W2035943929 cites W2015074490 @default.
• W2035943929 cites W2015803999 @default.
• W2035943929 cites W2016729734 @default.
• W2035943929 cites W2017509780 @default.
• W2035943929 cites W2019898105 @default.
• W2035943929 cites W2030261902 @default.
• W2035943929 cites W2043419172 @default.
• W2035943929 cites W2050083026 @default.
• W2035943929 cites W2055300965 @default.
• W2035943929 cites W2055615470 @default.
• W2035943929 cites W2057510253 @default.
• W2035943929 cites W2067229667 @default.
• W2035943929 cites W2067595418 @default.
• W2035943929 cites W2070065139 @default.
• W2035943929 cites W2070619662 @default.
• W2035943929 cites W2076079545 @default.
• W2035943929 cites W2076971907 @default.
• W2035943929 cites W2078594804 @default.
• W2035943929 cites W2097920834 @default.
• W2035943929 cites W2098776116 @default.
• W2035943929 cites W2107069418 @default.
• W2035943929 cites W2114119430 @default.
• W2035943929 cites W2137675092 @default.
• W2035943929 cites W2149790826 @default.
• W2035943929 cites W2156024250 @default.
• W2035943929 cites W2215736540 @default.
• W2035943929 cites W4251440173 @default.
• W2035943929 doi "https://doi.org/10.1016/j.freeradbiomed.2012.07.016" @default.
• W2035943929 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22884457" @default.
• W2035943929 hasPublicationYear "2012" @default.
• W2035943929 type Work @default.
• W2035943929 sameAs 2035943929 @default.
• W2035943929 citedByCount "29" @default.
• W2035943929 countsByYear W20359439292012 @default.
• W2035943929 countsByYear W20359439292013 @default.
• W2035943929 countsByYear W20359439292014 @default.
• W2035943929 countsByYear W20359439292015 @default.
• W2035943929 countsByYear W20359439292016 @default.
• W2035943929 countsByYear W20359439292019 @default.
• W2035943929 countsByYear W20359439292020 @default.
• W2035943929 countsByYear W20359439292021 @default.
• W2035943929 countsByYear W20359439292022 @default.
• W2035943929 countsByYear W20359439292023 @default.
• W2035943929 crossrefType "journal-article" @default.
• W2035943929 hasAuthorship W2035943929A5025701621 @default.
• W2035943929 hasAuthorship W2035943929A5029985039 @default.
• W2035943929 hasAuthorship W2035943929A5069317976 @default.
• W2035943929 hasConcept C162008176 @default.
• W2035943929 hasConcept C165069038 @default.
• W2035943929 hasConcept C178790620 @default.
• W2035943929 hasConcept C181199279 @default.
• W2035943929 hasConcept C185592680 @default.
• W2035943929 hasConcept C203014093 @default.
• W2035943929 hasConcept C203565725 @default.
• W2035943929 hasConcept C2776151105 @default.
• W2035943929 hasConcept C2776914184 @default.
• W2035943929 hasConcept C2777516457 @default.
• W2035943929 hasConcept C2777737464 @default.
• W2035943929 hasConcept C2778004101 @default.
• W2035943929 hasConcept C2778081958 @default.
• W2035943929 hasConcept C2778760513 @default.
• W2035943929 hasConcept C2780898351 @default.
• W2035943929 hasConcept C48349386 @default.
• W2035943929 hasConcept C538909803 @default.
• W2035943929 hasConcept C540031477 @default.
• W2035943929 hasConcept C55493867 @default.
• W2035943929 hasConcept C86803240 @default.
• W2035943929 hasConceptScore W2035943929C162008176 @default.
• W2035943929 hasConceptScore W2035943929C165069038 @default.
• W2035943929 hasConceptScore W2035943929C178790620 @default.
• W2035943929 hasConceptScore W2035943929C181199279 @default.
• W2035943929 hasConceptScore W2035943929C185592680 @default.

• next