Matches in SemOpenAlex for { <https://semopenalex.org/work/W2035965717> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2035965717 endingPage "235" @default.
- W2035965717 startingPage "229" @default.
- W2035965717 abstract "Most of our knowledge of the mammalian tyrosinase related protein (TRP) activities is derived from studies using murine melanoma models, such as B16 or Cloudman S-91 melanocytes. Owing to the high degree of homology between the murine and human enzymes, it has been assumed that their kinetic behaviour could be similar. However, the protein sequences at the metal binding sites of the murine and human enzymes show some differences of possible functional relevance. These differences are more significant in the metal-A site than in the metal-B site. By using three human melanoma cell lines (HBL, SCL, and BEU), we have studied the catalytic abilities of the human melanogenic enzymes in comparison to those obtained for the counterpart murine enzymes isolated from B16 melanoma. We have found that TRP2 extracted from all cell lines show dopachrome tautomerase activity, although the activity levels in human malignant melanocytes are much lower than in mouse cells. Reconstitution experiments of the human enzyme indicate that TRP2 has Zn at its metal binding-sites. Although mouse tyrosinase does not show DHICA oxidase activity, and this step of the melanogenesis pathway is specifically catalyzed by mouse TRP1, the human enzyme seems to recognize carboxylated indoles. Thus, human tyrosinase could display some residual DHICA oxidase activity, and the function of human TRP1 could differ from that of the murine protein. Attempts to clarify the nature of the metal cofactor in TRP1 were unsuccessful. The enzyme contains mostly Fe and Cu, but the reconstitution of the enzymatic activity from the apoprotein with these ions was not possible." @default.
- W2035965717 created "2016-06-24" @default.
- W2035965717 creator A5007483917 @default.
- W2035965717 creator A5022141490 @default.
- W2035965717 creator A5034634907 @default.
- W2035965717 creator A5040221388 @default.
- W2035965717 creator A5071967179 @default.
- W2035965717 creator A5072372960 @default.
- W2035965717 creator A5083106557 @default.
- W2035965717 date "1997-08-01" @default.
- W2035965717 modified "2023-10-17" @default.
- W2035965717 title "Comparison of TRPs From Murine and Human Malignant Melanocytes" @default.
- W2035965717 cites W1498389773 @default.
- W2035965717 cites W1514582998 @default.
- W2035965717 cites W1523767921 @default.
- W2035965717 cites W1536967003 @default.
- W2035965717 cites W1554730907 @default.
- W2035965717 cites W1591346049 @default.
- W2035965717 cites W1644644959 @default.
- W2035965717 cites W1964952893 @default.
- W2035965717 cites W1972863321 @default.
- W2035965717 cites W1986836408 @default.
- W2035965717 cites W1995042641 @default.
- W2035965717 cites W1995515070 @default.
- W2035965717 cites W2012480020 @default.
- W2035965717 cites W2021772147 @default.
- W2035965717 cites W2050291486 @default.
- W2035965717 cites W2061437892 @default.
- W2035965717 cites W2066849510 @default.
- W2035965717 cites W2075435354 @default.
- W2035965717 cites W2078587953 @default.
- W2035965717 cites W2080429461 @default.
- W2035965717 cites W2111486528 @default.
- W2035965717 cites W2152137853 @default.
- W2035965717 cites W2163410470 @default.
- W2035965717 cites W2165367433 @default.
- W2035965717 cites W2471820079 @default.
- W2035965717 cites W4238048551 @default.
- W2035965717 doi "https://doi.org/10.1111/j.1600-0749.1997.tb00489.x" @default.
- W2035965717 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9263330" @default.
- W2035965717 hasPublicationYear "1997" @default.
- W2035965717 type Work @default.
- W2035965717 sameAs 2035965717 @default.
- W2035965717 citedByCount "7" @default.
- W2035965717 countsByYear W20359657172017 @default.
- W2035965717 countsByYear W20359657172023 @default.
- W2035965717 crossrefType "journal-article" @default.
- W2035965717 hasAuthorship W2035965717A5007483917 @default.
- W2035965717 hasAuthorship W2035965717A5022141490 @default.
- W2035965717 hasAuthorship W2035965717A5034634907 @default.
- W2035965717 hasAuthorship W2035965717A5040221388 @default.
- W2035965717 hasAuthorship W2035965717A5071967179 @default.
- W2035965717 hasAuthorship W2035965717A5072372960 @default.
- W2035965717 hasAuthorship W2035965717A5083106557 @default.
- W2035965717 hasConcept C153911025 @default.
- W2035965717 hasConcept C181199279 @default.
- W2035965717 hasConcept C185592680 @default.
- W2035965717 hasConcept C2777658100 @default.
- W2035965717 hasConcept C2779769559 @default.
- W2035965717 hasConcept C502942594 @default.
- W2035965717 hasConcept C54355233 @default.
- W2035965717 hasConcept C55493867 @default.
- W2035965717 hasConcept C81885089 @default.
- W2035965717 hasConcept C86803240 @default.
- W2035965717 hasConcept C87554066 @default.
- W2035965717 hasConceptScore W2035965717C153911025 @default.
- W2035965717 hasConceptScore W2035965717C181199279 @default.
- W2035965717 hasConceptScore W2035965717C185592680 @default.
- W2035965717 hasConceptScore W2035965717C2777658100 @default.
- W2035965717 hasConceptScore W2035965717C2779769559 @default.
- W2035965717 hasConceptScore W2035965717C502942594 @default.
- W2035965717 hasConceptScore W2035965717C54355233 @default.
- W2035965717 hasConceptScore W2035965717C55493867 @default.
- W2035965717 hasConceptScore W2035965717C81885089 @default.
- W2035965717 hasConceptScore W2035965717C86803240 @default.
- W2035965717 hasConceptScore W2035965717C87554066 @default.
- W2035965717 hasIssue "4" @default.
- W2035965717 hasLocation W20359657171 @default.
- W2035965717 hasLocation W20359657172 @default.
- W2035965717 hasOpenAccess W2035965717 @default.
- W2035965717 hasPrimaryLocation W20359657171 @default.
- W2035965717 hasRelatedWork W1976813357 @default.
- W2035965717 hasRelatedWork W1985729602 @default.
- W2035965717 hasRelatedWork W2059166222 @default.
- W2035965717 hasRelatedWork W2065996563 @default.
- W2035965717 hasRelatedWork W2081684895 @default.
- W2035965717 hasRelatedWork W2084881622 @default.
- W2035965717 hasRelatedWork W2183553772 @default.
- W2035965717 hasRelatedWork W2944726932 @default.
- W2035965717 hasRelatedWork W3031721965 @default.
- W2035965717 hasRelatedWork W4294295994 @default.
- W2035965717 hasVolume "10" @default.
- W2035965717 isParatext "false" @default.
- W2035965717 isRetracted "false" @default.
- W2035965717 magId "2035965717" @default.
- W2035965717 workType "article" @default.