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• W2036007066 abstract "Seven myosin heavy chains (MyHC) are expressed in mammalian skeletal muscle in spatially and temporally regulated patterns. The timing, distribution, and quantitation of MyHC expression during development and early postnatal life of the mouse are reported here. The three adult fast MyHC RNAs (IIa, IIb, and IId/x) are expressed in the mouse embryo and each mRNA has a distinct temporal and spatial distribution. In situ hybridization analysis demonstrates expression of IIb mRNA by 14.5 dpc, which proceeds developmentally in a rostral to caudal pattern. IId/x and IIa mRNAs are detectable 2 days later. Ribonuclease protection assays demonstrate that the three adult fast genes are expressed at approximately equal levels relative to each other in the embryo but at quite low levels relative to the two developmental isoforms, embryonic and perinatal. Just after birth major changes in the relative proportions of different MyHC RNAs and protein occur. In all cases, RNA expression and protein expression appear coincident. The changes in MyHC RNA and protein expression are distinct in different muscles and are restricted in some cases to particular regions of the muscle and do not always reflect their distribution in the adult." @default.
• W2036007066 created "2016-06-24" @default.
• W2036007066 creator A5021010763 @default.
• W2036007066 creator A5052631065 @default.
• W2036007066 creator A5079901423 @default.
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• W2036007066 date "1999-12-01" @default.
• W2036007066 modified "2023-09-26" @default.
• W2036007066 title "Spatial and Temporal Changes in Myosin Heavy Chain Gene Expression in Skeletal Muscle Development" @default.
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• W2036007066 doi "https://doi.org/10.1006/dbio.1999.9488" @default.
• W2036007066 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10588881" @default.
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