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• W2036018049 abstract "The influence of early hypothyroidism on the concentration and biochemical properties of soluble and particulate tubulin from the cerebral cortex and cerebellum was investigated during development in the rat. Cellular soluble tubulin concentration (pmol colchicine bound/μg DNA) was approx 16% lower in both brain areas of hypothyroid animals compared to controls at 25 days of age. No effect of thyroid hormone deficiency was observed when tubulin concentration was expressed in terms of tissue protein or weight. The particulate tubulin concentration was approx 20% lower in the cerebral cortex of 25-day-old hypothyroid rats although the distribution of tubulin between soluble and particulate fractions was similar to controls. The incorporation of [14C]leucine into cerebral cortical tubulin in vitro (c.p.m. in tubulin/c.p.m. in total protein) was not significantly altered by the hormonal deficiency. Thus there was no apparent evidence of a selective defect in tubulin synthesis. Tubulin from hypothyroid rats behaved similarly to control samples with respect to the effects of pharmacological agents and temperature, lability of binding, chromatographic profile and electrophoretic mobility on sodium dodecyl sulfate polyacrylamide gels." @default.
• W2036018049 created "2016-06-24" @default.
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• W2036018049 date "1978-01-01" @default.
• W2036018049 modified "2023-10-17" @default.
• W2036018049 title "QUANTITATION AND CHARACTERIZATION OF BRAIN TUBULIN (COLCHICINE-BINDING ACTIVITY) IN DEVELOPING HYPOTHYROID RATS" @default.
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• W2036018049 doi "https://doi.org/10.1111/j.1471-4159.1978.tb07057.x" @default.
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