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- W2036065634 abstract "Abstract Stable isotope labeling by SILAC‐based quantitative proteomics analysis provides an unprecedented tool for the study of mechanisms underlying the self‐renewal and differentiation of human embryonic stem cells (hESCs). While we recently reported a chemically defined SILAC culture system specific for a rare cell proteomic reactor (R. Tian et al., Mol. Cell. Proteomics 2011, 10 , M110.000679), total hESC yield, prolonged self‐renewal capacity (i.e.<12 days), and laborious procedure remain substantial hurdles for its conventional application in hESC studies. Here, we devised an enhanced SILAC culture system consisting of a new chemically defined SILAC‐medium and a novel culture protocol. As a result, with much less culture maneuvers, approximately 40‐fold greater hESCs were produced than the system reported previously. Moreover, the enhanced SILAC culture system was sufficient to support the self‐renewal of hESCs for >60 days and was also highly reproducible. As such, it provides a new platform that can be readily adapted by general laboratory for further comprehensive SILAC‐based proteomics analysis of hESCs and induced pluripotent stem cells." @default.
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- W2036065634 date "2011-08-31" @default.
- W2036065634 modified "2023-10-07" @default.
- W2036065634 title "An enhanced chemically defined SILAC culture system for quantitative proteomics study of human embryonic stem cells" @default.
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- W2036065634 doi "https://doi.org/10.1002/pmic.201100052" @default.
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