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- W2036075900 abstract "Affinity chromatography on concanavalin A-Sepharose is a time saving step in both large and small scale isolations of the bovine pituitary glycoprotein hormones. After ion-exchange chromatography, the final yield of purified lutropin is 40-50% of material in starting concentrates and of purified thyrotropin is approximately 20%. The final products have the same electrophoretic and immunological properties and amino acid compositions as previous preparations. Less than 3% of the immunoreactive lutropin, follitropin and thyrotropin are present as non-glycosylated forms in either crude pituitary extracts or concentrates. Thyrotropin and follitropin elute from the immobilized lectin as a single fraction, whereas lutropin separates into two glycosylated fractions. Gel filtration of both crude extracts and the glycoprotein fractions shows that less than 5% of the immunoreactivity of the hormones is present as material of apparently high molecular weight. Substantial alpha subunit immunoreactivity, however, is in three fractions (as found by others in human pituitary extracts) corresponding to high molecular weight material (7%), intact hormones (46%) and free subunit (47%)." @default.
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- W2036075900 date "1978-04-01" @default.
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- W2036075900 title "Sepharose-linked concanavalin A in the purification and characterization of glycoprotein hormones of the bovine pituitary" @default.
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- W2036075900 doi "https://doi.org/10.1016/0005-2795(78)90383-5" @default.
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