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• W2036096957 startingPage "15908" @default.
• W2036096957 abstract "The C+1/A+72 base pair at the top of the acceptor stem of Escherichia coli tRNAfMet accounts for several of the specialized roles of this tRNA in translation initiation. According to the rules of RNA substrate recognition by RNase P, the C+1/A+72 pair is likely to disfavor the 5′-maturation of pre-tRNAfMet. Indeed, in contrast to other E. coli tRNA species, tRNAfMet was not properly matured when overproduced from a multicopy expression vector. Half of the recovered tRNAfMet retained an extension at the 5′ side. Such a defect of tRNAfMet processing could be cured by changing bases C+1 and A+72 by a Watson-Crick base pair or by non-paired bases, provided one of them was a G. It could also be compensated by either (i) overexpression of RNase P or (ii) introduction within the plasmid of one out of the three 5′-flanking sequences naturally occurring in the four E. coli tRNAfMet genes. The effect of these flanking sequences on the maturation of tRNAfMet could be accounted for by the presence of a G located 2 bases upstream from C+1. Notably, this G is the only residue that is conserved in the 5′-flanking sequences of all four E. coli tRNAfMet genes. The C+1/A+72 base pair at the top of the acceptor stem of Escherichia coli tRNAfMet accounts for several of the specialized roles of this tRNA in translation initiation. According to the rules of RNA substrate recognition by RNase P, the C+1/A+72 pair is likely to disfavor the 5′-maturation of pre-tRNAfMet. Indeed, in contrast to other E. coli tRNA species, tRNAfMet was not properly matured when overproduced from a multicopy expression vector. Half of the recovered tRNAfMet retained an extension at the 5′ side. Such a defect of tRNAfMet processing could be cured by changing bases C+1 and A+72 by a Watson-Crick base pair or by non-paired bases, provided one of them was a G. It could also be compensated by either (i) overexpression of RNase P or (ii) introduction within the plasmid of one out of the three 5′-flanking sequences naturally occurring in the four E. coli tRNAfMet genes. The effect of these flanking sequences on the maturation of tRNAfMet could be accounted for by the presence of a G located 2 bases upstream from C+1. Notably, this G is the only residue that is conserved in the 5′-flanking sequences of all four E. coli tRNAfMet genes." @default.
• W2036096957 created "2016-06-24" @default.
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• W2036096957 date "1995-06-01" @default.
• W2036096957 modified "2023-10-18" @default.
• W2036096957 title "Maturation of Pre-tRNAfMet by Escherichia coli RNase P Is Specified by a Guanosine of the 5′-Flanking Sequence" @default.
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• W2036096957 doi "https://doi.org/10.1074/jbc.270.26.15908" @default.
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