Matches in SemOpenAlex for { <https://semopenalex.org/work/W2036097300> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2036097300 endingPage "1971" @default.
- W2036097300 startingPage "1963" @default.
- W2036097300 abstract "Cystathionine beta-synthase (CBS) effects the condensation of l-serine with l-homocysteine to form l-cystathionine. A series of active-site mutants, T81A, S82A, T85A, Q157A/E/H, and Y158F, was constructed to investigate effects on catalysis and reaction specificity in yeast CBS (yCBS). The effects of these mutations on the k(cat)/K(m)(L-Ser) for the beta-replacement reaction range from a reduction of only 3-fold for Y158F to below detectable levels for the Q157A and Q157E mutants. The order of importance of these residues to the beta-replacement reaction is Gln157 >or= Thr81 > Ser82 > Thr85 approximately Tyr158. All seven of the mutant enzymes catalyze a competing beta-elimination reaction, in which L-Ser is hydrolyzed to NH(3) and pyruvate. The ping-pong mechanism of CBS was thus expanded to include the latter reaction for these mutants. This activity is not detectable for wild-type yCBS, suggesting that the mutations result in a shift in the equilibrium between the open and the closed conformations of the active site of yCBS-substrate complexes. The Q157H and Y158F mutants additionally suffer suicide inhibition via a mechanism in which the released aminoacrylate intermediate covalently attacks the internal aldimine of the enzyme." @default.
- W2036097300 created "2016-06-24" @default.
- W2036097300 creator A5013024704 @default.
- W2036097300 creator A5077016752 @default.
- W2036097300 date "2004-01-27" @default.
- W2036097300 modified "2023-10-16" @default.
- W2036097300 title "Role of Active-Site Residues Thr81, Ser82, Thr85, Gln157, and Tyr158 inYeast Cystathionine β-Synthase Catalysis and Reaction Specificity" @default.
- W2036097300 cites W1516448862 @default.
- W2036097300 cites W1563387341 @default.
- W2036097300 cites W1602279263 @default.
- W2036097300 cites W1972135076 @default.
- W2036097300 cites W1982341626 @default.
- W2036097300 cites W2003300391 @default.
- W2036097300 cites W2007549861 @default.
- W2036097300 cites W2011269229 @default.
- W2036097300 cites W2035943254 @default.
- W2036097300 cites W2047692305 @default.
- W2036097300 cites W2090421667 @default.
- W2036097300 cites W2118972497 @default.
- W2036097300 cites W2131942711 @default.
- W2036097300 doi "https://doi.org/10.1021/bi035496m" @default.
- W2036097300 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14967036" @default.
- W2036097300 hasPublicationYear "2004" @default.
- W2036097300 type Work @default.
- W2036097300 sameAs 2036097300 @default.
- W2036097300 citedByCount "18" @default.
- W2036097300 countsByYear W20360973002014 @default.
- W2036097300 countsByYear W20360973002015 @default.
- W2036097300 countsByYear W20360973002017 @default.
- W2036097300 countsByYear W20360973002019 @default.
- W2036097300 countsByYear W20360973002020 @default.
- W2036097300 crossrefType "journal-article" @default.
- W2036097300 hasAuthorship W2036097300A5013024704 @default.
- W2036097300 hasAuthorship W2036097300A5077016752 @default.
- W2036097300 hasConcept C104317684 @default.
- W2036097300 hasConcept C143065580 @default.
- W2036097300 hasConcept C161790260 @default.
- W2036097300 hasConcept C16525657 @default.
- W2036097300 hasConcept C181199279 @default.
- W2036097300 hasConcept C185592680 @default.
- W2036097300 hasConcept C187566844 @default.
- W2036097300 hasConcept C18903297 @default.
- W2036097300 hasConcept C2776414213 @default.
- W2036097300 hasConcept C2777289219 @default.
- W2036097300 hasConcept C2779201268 @default.
- W2036097300 hasConcept C41183919 @default.
- W2036097300 hasConcept C55493867 @default.
- W2036097300 hasConcept C67524406 @default.
- W2036097300 hasConcept C71240020 @default.
- W2036097300 hasConcept C86803240 @default.
- W2036097300 hasConceptScore W2036097300C104317684 @default.
- W2036097300 hasConceptScore W2036097300C143065580 @default.
- W2036097300 hasConceptScore W2036097300C161790260 @default.
- W2036097300 hasConceptScore W2036097300C16525657 @default.
- W2036097300 hasConceptScore W2036097300C181199279 @default.
- W2036097300 hasConceptScore W2036097300C185592680 @default.
- W2036097300 hasConceptScore W2036097300C187566844 @default.
- W2036097300 hasConceptScore W2036097300C18903297 @default.
- W2036097300 hasConceptScore W2036097300C2776414213 @default.
- W2036097300 hasConceptScore W2036097300C2777289219 @default.
- W2036097300 hasConceptScore W2036097300C2779201268 @default.
- W2036097300 hasConceptScore W2036097300C41183919 @default.
- W2036097300 hasConceptScore W2036097300C55493867 @default.
- W2036097300 hasConceptScore W2036097300C67524406 @default.
- W2036097300 hasConceptScore W2036097300C71240020 @default.
- W2036097300 hasConceptScore W2036097300C86803240 @default.
- W2036097300 hasIssue "7" @default.
- W2036097300 hasLocation W20360973001 @default.
- W2036097300 hasLocation W20360973002 @default.
- W2036097300 hasOpenAccess W2036097300 @default.
- W2036097300 hasPrimaryLocation W20360973001 @default.
- W2036097300 hasRelatedWork W1973134985 @default.
- W2036097300 hasRelatedWork W2021396179 @default.
- W2036097300 hasRelatedWork W2036097300 @default.
- W2036097300 hasRelatedWork W2036177646 @default.
- W2036097300 hasRelatedWork W2087140484 @default.
- W2036097300 hasRelatedWork W258580054 @default.
- W2036097300 hasRelatedWork W2753827332 @default.
- W2036097300 hasRelatedWork W3084057161 @default.
- W2036097300 hasRelatedWork W3104983722 @default.
- W2036097300 hasRelatedWork W3136650548 @default.
- W2036097300 hasVolume "43" @default.
- W2036097300 isParatext "false" @default.
- W2036097300 isRetracted "false" @default.
- W2036097300 magId "2036097300" @default.
- W2036097300 workType "article" @default.