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• W2036257711 endingPage "1364" @default.
• W2036257711 startingPage "1359" @default.
• W2036257711 abstract "This study re-evaluated a protocol for cryopreservation of canine semen. Semen from 4 beagle dogs was pooled, concentrated by centrifugation and adjusted to increasing sperm concentrations by adding back seminal plasma. The prepared or original semen was diluted with an extender (Egg yolk-Tris-citrate-glucose) and cooled to 4°C (cooling), followed by a second dilution with the same extender including glycerol, equilibrated at 4°C (equilibration), then stored in liquid nitrogen. The semen was diluted for frozen samples having a fixed sperm concentration with increasing dilution rates or for those having the reverse combinations. Various dilution rates of 2.5-10 folds or sperm concentrations of 0.25-2.5 × 108/ml had no significant effect on post-thaw sperm characteristics. When cooling was done for different times (0-26 hr) with glycerol equilibration for 1 hr, post-thaw characteristics were better at 2 and 3 hr of cooling, while various times for equilibration (0-4 hr) with cooling for 3 hr had no effect. These results suggest that different dilution rates and sperm concentrations within the ranges tested may not affect the post-thaw sperm characterisitics and that sufficient time for cooling may be essential but a specific equilibration time may not necessarily be required." @default.
• W2036257711 created "2016-06-24" @default.
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• W2036257711 date "2004-01-01" @default.
• W2036257711 modified "2023-09-27" @default.
• W2036257711 title "Effects of Final Dilution Rate, Sperm Concentration and Times for Cooling and Glycerol Equilibration on Post-Thaw Characteristics of Canine Spermatozoa" @default.
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• W2036257711 doi "https://doi.org/10.1292/jvms.66.1359" @default.
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• W2036257711 hasPublicationYear "2004" @default.
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