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• W2036366956 endingPage "3475" @default.
• W2036366956 startingPage "3471" @default.
• W2036366956 abstract "We have cloned a positive regulatory gene ( toxR ) from Vibrio cholerae that controls cholera toxin transcription. This was done by first constructing a genetic fusion consisting of the lacZ gene fused to the promoter of the cholera toxin operon ctxAB . This operon fusion was used to screen a V. cholerae genomic library for genes that could activate the ctx promoter in Escherichia coli. This method allowed the identification of a gene, toxR , that increases ctx expression by more than 100-fold. Complementation analysis indicated that certain hypotoxinogenic mutants of V. cholerae 569B probably have mutations in the toxR gene. Southern blot analysis suggests that all V. cholerae, including nontoxinogenic strains, have the toxR gene. Moreover, nontoxinogenic strains not only lack the structural genes for cholera toxin but also sequences associated with the larger 7-kilobase ctx genetic element." @default.
• W2036366956 created "2016-06-24" @default.
• W2036366956 creator A5045253972 @default.
• W2036366956 creator A5047063103 @default.
• W2036366956 date "1984-06-01" @default.
• W2036366956 modified "2023-09-27" @default.
• W2036366956 title "Synthesis of cholera toxin is positively regulated at the transcriptional level by toxR." @default.
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• W2036366956 doi "https://doi.org/10.1073/pnas.81.11.3471" @default.
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