FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2036409857> ?p ?o ?g. }

• W2036409857 endingPage "8114" @default.
• W2036409857 startingPage "8108" @default.
• W2036409857 abstract "In this study we have examined the role of insulin-like growth factor-II (IGF-II) in the differentiation of the CaCo-2 human colon carcinoma cell line. We have shown previously that IGF-II is an autocrine growth factor for CaCo-2 cells. IGF-II expression is high in proliferating, undifferentiated CaCo-2 cells and markedly decreases when cells become confluent and start to differentiate. To evaluate whether differentiation of CaCo-2 cells depends on an IGF-II related pathway, we treated cells with a blocking antibody to the IGF-I receptor that mediates most IGF-II biological effects. Treatment of preconfluent CaCo-2 cells with this antibody decreased by 40% autonomous cell proliferation and induced differentiation as shown by an increase in sucrase isomaltase activity and apolipoprotein A-I (apoA-I) mRNA levels. To examine the significance of autocrine IGF-II production in CaCo-2 cell differentiation, we generated stable CaCo-2 cell lines that constitutively express rat IGF-II under the control of a Rous sarcoma virus promoter. Sustained expression of IGF-II resulted in: (a) increased proliferative rate; (b) high IGF-I receptor number, even after reaching confluence; (c) increased capability of anchorage-independent growth; (d) inhibition of the expression of apoA-I and SI mRNAs. Analysis of several independent IGF-II-transfected clones showed an inverse correlation between IGF-II mRNA levels and expression of the differentiation markers, the cells expressing the higher levels of the transfected IGF-II being the less differentiated ones. Our data suggest that perturbation of IGF-II-mediated cell proliferation interferes with the enterocyte-like differentiation pathway of CaCo-2 cells. In this study we have examined the role of insulin-like growth factor-II (IGF-II) in the differentiation of the CaCo-2 human colon carcinoma cell line. We have shown previously that IGF-II is an autocrine growth factor for CaCo-2 cells. IGF-II expression is high in proliferating, undifferentiated CaCo-2 cells and markedly decreases when cells become confluent and start to differentiate. To evaluate whether differentiation of CaCo-2 cells depends on an IGF-II related pathway, we treated cells with a blocking antibody to the IGF-I receptor that mediates most IGF-II biological effects. Treatment of preconfluent CaCo-2 cells with this antibody decreased by 40% autonomous cell proliferation and induced differentiation as shown by an increase in sucrase isomaltase activity and apolipoprotein A-I (apoA-I) mRNA levels. To examine the significance of autocrine IGF-II production in CaCo-2 cell differentiation, we generated stable CaCo-2 cell lines that constitutively express rat IGF-II under the control of a Rous sarcoma virus promoter. Sustained expression of IGF-II resulted in: (a) increased proliferative rate; (b) high IGF-I receptor number, even after reaching confluence; (c) increased capability of anchorage-independent growth; (d) inhibition of the expression of apoA-I and SI mRNAs. Analysis of several independent IGF-II-transfected clones showed an inverse correlation between IGF-II mRNA levels and expression of the differentiation markers, the cells expressing the higher levels of the transfected IGF-II being the less differentiated ones. Our data suggest that perturbation of IGF-II-mediated cell proliferation interferes with the enterocyte-like differentiation pathway of CaCo-2 cells." @default.
• W2036409857 created "2016-06-24" @default.
• W2036409857 creator A5032947684 @default.
• W2036409857 creator A5042222956 @default.
• W2036409857 creator A5045864473 @default.
• W2036409857 creator A5057998269 @default.
• W2036409857 creator A5083659627 @default.
• W2036409857 creator A5006326279 @default.
• W2036409857 date "1996-04-01" @default.
• W2036409857 modified "2023-09-28" @default.
• W2036409857 title "Constitutive Insulin-like Growth Factor-II Expression Interferes with the Enterocyte-like Differentiation of CaCo-2 Cells" @default.
• W2036409857 cites W1492325161 @default.
• W2036409857 cites W1499890190 @default.
• W2036409857 cites W1524183923 @default.
• W2036409857 cites W159198934 @default.
• W2036409857 cites W1607586094 @default.
• W2036409857 cites W170591362 @default.
• W2036409857 cites W1973500578 @default.
• W2036409857 cites W1974540651 @default.
• W2036409857 cites W1977761121 @default.
• W2036409857 cites W1989119084 @default.
• W2036409857 cites W1995453259 @default.
• W2036409857 cites W1996131974 @default.
• W2036409857 cites W2007573212 @default.
• W2036409857 cites W2008954530 @default.
• W2036409857 cites W2009440060 @default.
• W2036409857 cites W2012083381 @default.
• W2036409857 cites W2015760663 @default.
• W2036409857 cites W2016693389 @default.
• W2036409857 cites W2017615910 @default.
• W2036409857 cites W2043813981 @default.
• W2036409857 cites W2058723098 @default.
• W2036409857 cites W2072305097 @default.
• W2036409857 cites W2076857471 @default.
• W2036409857 cites W2085063690 @default.
• W2036409857 cites W2092143533 @default.
• W2036409857 cites W2124416653 @default.
• W2036409857 cites W2127006469 @default.
• W2036409857 cites W2129152034 @default.
• W2036409857 cites W2139070258 @default.
• W2036409857 cites W2186770219 @default.
• W2036409857 cites W2008310327 @default.
• W2036409857 doi "https://doi.org/10.1074/jbc.271.14.8108" @default.
• W2036409857 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8626497" @default.
• W2036409857 hasPublicationYear "1996" @default.
• W2036409857 type Work @default.
• W2036409857 sameAs 2036409857 @default.
• W2036409857 citedByCount "37" @default.
• W2036409857 countsByYear W20364098572015 @default.
• W2036409857 crossrefType "journal-article" @default.
• W2036409857 hasAuthorship W2036409857A5006326279 @default.
• W2036409857 hasAuthorship W2036409857A5032947684 @default.
• W2036409857 hasAuthorship W2036409857A5042222956 @default.
• W2036409857 hasAuthorship W2036409857A5045864473 @default.
• W2036409857 hasAuthorship W2036409857A5057998269 @default.
• W2036409857 hasAuthorship W2036409857A5083659627 @default.
• W2036409857 hasBestOaLocation W20364098571 @default.
• W2036409857 hasConcept C104317684 @default.
• W2036409857 hasConcept C128240485 @default.
• W2036409857 hasConcept C134018914 @default.
• W2036409857 hasConcept C148738053 @default.
• W2036409857 hasConcept C153911025 @default.
• W2036409857 hasConcept C170493617 @default.
• W2036409857 hasConcept C2775960820 @default.
• W2036409857 hasConcept C2779069268 @default.
• W2036409857 hasConcept C2780689927 @default.
• W2036409857 hasConcept C54009773 @default.
• W2036409857 hasConcept C54355233 @default.
• W2036409857 hasConcept C55493867 @default.
• W2036409857 hasConcept C81885089 @default.
• W2036409857 hasConcept C86803240 @default.
• W2036409857 hasConcept C95444343 @default.
• W2036409857 hasConceptScore W2036409857C104317684 @default.
• W2036409857 hasConceptScore W2036409857C128240485 @default.
• W2036409857 hasConceptScore W2036409857C134018914 @default.
• W2036409857 hasConceptScore W2036409857C148738053 @default.
• W2036409857 hasConceptScore W2036409857C153911025 @default.
• W2036409857 hasConceptScore W2036409857C170493617 @default.
• W2036409857 hasConceptScore W2036409857C2775960820 @default.
• W2036409857 hasConceptScore W2036409857C2779069268 @default.
• W2036409857 hasConceptScore W2036409857C2780689927 @default.
• W2036409857 hasConceptScore W2036409857C54009773 @default.
• W2036409857 hasConceptScore W2036409857C54355233 @default.
• W2036409857 hasConceptScore W2036409857C55493867 @default.
• W2036409857 hasConceptScore W2036409857C81885089 @default.
• W2036409857 hasConceptScore W2036409857C86803240 @default.
• W2036409857 hasConceptScore W2036409857C95444343 @default.
• W2036409857 hasIssue "14" @default.
• W2036409857 hasLocation W20364098571 @default.
• W2036409857 hasOpenAccess W2036409857 @default.
• W2036409857 hasPrimaryLocation W20364098571 @default.
• W2036409857 hasRelatedWork W1565618380 @default.
• W2036409857 hasRelatedWork W1973500578 @default.
• W2036409857 hasRelatedWork W2008457395 @default.
• W2036409857 hasRelatedWork W2080009865 @default.
• W2036409857 hasRelatedWork W2105749423 @default.
• W2036409857 hasRelatedWork W2118032571 @default.
• W2036409857 hasRelatedWork W2130733926 @default.

• next