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• W2036413396 abstract "Theileria parasites transform bovine leukocytes and induce uncontrolled lymphoproliferation only in the macroschizont stage of their life cycle. The isolation of highly purified stage-specific parasite RNA and proteins is an essential prerequisite when studying the Theileria-host relationship. We therefore improved a protocol based on the cytolytic bacterial toxin aerolysin by taking advantage of the microtubule inhibitor nocodazole. In this report we describe that nocodazole-mediated separation of the parasite from the host cell microtubule network was used with success to improve quantity and quality of purified parasites. We furthermore show that nocodazole is a useful tool to study cell cycle checkpoints due to its capacity to induce reversible cell cycle arrest in Theileria-infected B cells." @default.
• W2036413396 created "2016-06-24" @default.
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• W2036413396 date "1999-12-01" @default.
• W2036413396 modified "2023-09-27" @default.
• W2036413396 title "The use of nocodazole in cell cycle analysis and parasite purification from Theileria parva-infected B cells" @default.
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• W2036413396 doi "https://doi.org/10.1016/s1286-4579(99)00244-0" @default.
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