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- W2036478400 abstract "The kinetic behavior of five models for biological transport, only one of which is based on the classical carrier mechanism, is investigated. All give hyperbolic substrate saturation curves in accord with experimental observations on many systems. Several simple kinetic tests with substrates and competitive inhibitors serve to exclude or confirm proposed models. The tests involve measuring rates of efflux of radioactive substrate in the presence of (i) a competitive inhibitor outside the cell; (ii) inhibitor inside and outside; and (iii) unlabeled substrate outside. Rules for testing hypothetical mechanisms are presented in tables which may be consulted directly, disregarding the mathematical derivation." @default.
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- W2036478400 date "1978-10-01" @default.
- W2036478400 modified "2023-10-06" @default.
- W2036478400 title "Testing transport models with substrates and reversible inhibitors" @default.
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- W2036478400 doi "https://doi.org/10.1016/0005-2736(78)90120-7" @default.
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