FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2036485905> ?p ?o ?g. }

• W2036485905 endingPage "68" @default.
• W2036485905 startingPage "58" @default.
• W2036485905 abstract "Comprehensive analysis of complex proteomes requires prefractionation of samples prior to two-dimensional gel electrophoresis (2-DE). This study demonstrates the utility of using a high resolution sample prefractionation method and slightly overlapping narrow pH range two-dimensional gel electrophoresis to enhance quantitative comparisons of complex proteomes. A key feature of this strategy is to prefractionate samples into a few well-defined pools using microscale solution isoelectric focusing (νsol-IEF) prior to 2-DE protein analysis. Sample prefractionation is achieved using a series of tandem small volume chambers (500 νL) separated by thin membranes containing immobilines at specific pH’s. The resulting well-resolved fractionated samples are optimally separated on a series of slightly overlapping narrow pH range immobilized pH gradient (IPG) gels, which are approximately 0.1 pH units wider than the νsol-IEF fractionated pools. When νsol-IEF prefractionation was applied to proteome analyses of mouse serum, it resulted in the capacity to separate much higher protein loads on narrow pH range IPG gels while retaining good resolution and spot recovery. More importantly, the prefractionation of serum greatly enhanced the ability to detect low abundance proteins, because major interfering proteins were removed from most fractions. At least 6- to 30-fold higher protein loads were possible for nonalbumin fractions on narrow pH range IPG gels. The dynamic range of protein detection is substantially increased since higher protein loads can be applied to narrow pH range 2-DE gels, and duplicate gels can be stained with colloidal Coomassie and silver stains for quantitation of abundant and minor proteins, respectively. Finally, the ability to effectively fractionate complex proteomes into very narrow ranges (< 0.5 pH units) strongly suggests that νsol-IEF could be used to prefractionate complex samples for subsequent direct analysis by liquid chromatography-tandem mass spectrometry methods as an alternative to using overlapping narrow pH range 2-DE gels." @default.
• W2036485905 created "2016-06-24" @default.
• W2036485905 creator A5039555481 @default.
• W2036485905 creator A5070183118 @default.
• W2036485905 date "2002-01-01" @default.
• W2036485905 modified "2023-10-16" @default.
• W2036485905 title "Comprehensive analysis of complex proteomes using microscale solution isoelectrofocusing prior to narrow pH range two-dimensional electrophoresis" @default.
• W2036485905 cites W1597678601 @default.
• W2036485905 cites W170585602 @default.
• W2036485905 cites W1972807195 @default.
• W2036485905 cites W1975366308 @default.
• W2036485905 cites W1978038810 @default.
• W2036485905 cites W1991613592 @default.
• W2036485905 cites W1998907788 @default.
• W2036485905 cites W2000225365 @default.
• W2036485905 cites W2007629654 @default.
• W2036485905 cites W2007886870 @default.
• W2036485905 cites W2008232473 @default.
• W2036485905 cites W2046176730 @default.
• W2036485905 cites W2065188739 @default.
• W2036485905 cites W2073304019 @default.
• W2036485905 cites W2092924155 @default.
• W2036485905 cites W2109978179 @default.
• W2036485905 cites W2111208057 @default.
• W2036485905 cites W2150389507 @default.
• W2036485905 cites W4296926797 @default.
• W2036485905 doi "https://doi.org/10.1002/1615-9861(200201)2:1<58::aid-prot58>3.0.co;2-g" @default.
• W2036485905 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11788992" @default.
• W2036485905 hasPublicationYear "2002" @default.
• W2036485905 type Work @default.
• W2036485905 sameAs 2036485905 @default.
• W2036485905 citedByCount "130" @default.
• W2036485905 countsByYear W20364859052012 @default.
• W2036485905 countsByYear W20364859052013 @default.
• W2036485905 countsByYear W20364859052014 @default.
• W2036485905 countsByYear W20364859052015 @default.
• W2036485905 countsByYear W20364859052016 @default.
• W2036485905 countsByYear W20364859052017 @default.
• W2036485905 countsByYear W20364859052020 @default.
• W2036485905 crossrefType "journal-article" @default.
• W2036485905 hasAuthorship W2036485905A5039555481 @default.
• W2036485905 hasAuthorship W2036485905A5070183118 @default.
• W2036485905 hasConcept C104317684 @default.
• W2036485905 hasConcept C104397665 @default.
• W2036485905 hasConcept C113196181 @default.
• W2036485905 hasConcept C138268822 @default.
• W2036485905 hasConcept C145420912 @default.
• W2036485905 hasConcept C154337534 @default.
• W2036485905 hasConcept C154945302 @default.
• W2036485905 hasConcept C179428855 @default.
• W2036485905 hasConcept C181199279 @default.
• W2036485905 hasConcept C185592680 @default.
• W2036485905 hasConcept C2778576202 @default.
• W2036485905 hasConcept C2780761556 @default.
• W2036485905 hasConcept C33923547 @default.
• W2036485905 hasConcept C40250595 @default.
• W2036485905 hasConcept C41008148 @default.
• W2036485905 hasConcept C43617362 @default.
• W2036485905 hasConcept C46111723 @default.
• W2036485905 hasConcept C47793336 @default.
• W2036485905 hasConcept C50476208 @default.
• W2036485905 hasConcept C54355233 @default.
• W2036485905 hasConcept C55493867 @default.
• W2036485905 hasConcept C74864618 @default.
• W2036485905 hasConcept C86803240 @default.
• W2036485905 hasConceptScore W2036485905C104317684 @default.
• W2036485905 hasConceptScore W2036485905C104397665 @default.
• W2036485905 hasConceptScore W2036485905C113196181 @default.
• W2036485905 hasConceptScore W2036485905C138268822 @default.
• W2036485905 hasConceptScore W2036485905C145420912 @default.
• W2036485905 hasConceptScore W2036485905C154337534 @default.
• W2036485905 hasConceptScore W2036485905C154945302 @default.
• W2036485905 hasConceptScore W2036485905C179428855 @default.
• W2036485905 hasConceptScore W2036485905C181199279 @default.
• W2036485905 hasConceptScore W2036485905C185592680 @default.
• W2036485905 hasConceptScore W2036485905C2778576202 @default.
• W2036485905 hasConceptScore W2036485905C2780761556 @default.
• W2036485905 hasConceptScore W2036485905C33923547 @default.
• W2036485905 hasConceptScore W2036485905C40250595 @default.
• W2036485905 hasConceptScore W2036485905C41008148 @default.
• W2036485905 hasConceptScore W2036485905C43617362 @default.
• W2036485905 hasConceptScore W2036485905C46111723 @default.
• W2036485905 hasConceptScore W2036485905C47793336 @default.
• W2036485905 hasConceptScore W2036485905C50476208 @default.
• W2036485905 hasConceptScore W2036485905C54355233 @default.
• W2036485905 hasConceptScore W2036485905C55493867 @default.
• W2036485905 hasConceptScore W2036485905C74864618 @default.
• W2036485905 hasConceptScore W2036485905C86803240 @default.
• W2036485905 hasIssue "1" @default.
• W2036485905 hasLocation W20364859051 @default.
• W2036485905 hasLocation W20364859052 @default.
• W2036485905 hasOpenAccess W2036485905 @default.
• W2036485905 hasPrimaryLocation W20364859051 @default.
• W2036485905 hasRelatedWork W2036485905 @default.
• W2036485905 hasRelatedWork W2036791348 @default.
• W2036485905 hasRelatedWork W2078609312 @default.
• W2036485905 hasRelatedWork W2344455108 @default.
• W2036485905 hasRelatedWork W2356110440 @default.

• next