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- W2036502140 abstract "The osmotic potential for swelling of the brain was determined using feline cerebral tissue blocks as osmometers in deoxygenated Ringer's solution media containing various concentrations of the impermeant solute, polyethylene glycol 6000. The degree of swelling was determined gravimetrically. Curves showing the relationship between the volume change and glycol concentration were almost linear except for a slight S-shape deviation at about the zero-change level. The critical concentration at which the tissue blocks in Ringer's solution exhibited no volume change was 72 g/liter, from which the osmotic potential was estimated as 578 mm Hg. This high osmotic potential was attained within 30 min of anoxia, and maintained 12 h with a subsequent gradual decay. Analysis of concentration changes in small ions and proteins in the media during incubation of the tissue blocks revealed that the increased osmotic potential accompanied influxes of Na+ and Cl− into the tissue blocks and effluxes of K+ and proteins. The concentration changes in K+ and proteins showed a statistically significant correlation. On the basis of quantitative analyses of these results, it is considered that the increased osmotic potential was caused not only by changes in the membrane permeability of the cells but also by physicochemical changes in intracellular proteins. After ischemic anoxia of the brain in situ, the huge osmotic potential of approximately 600 mm Hg inherent in the tissue would become apparent as an imbibing force for the development of brain edema and swelling." @default.
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- W2036502140 date "1979-07-01" @default.
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- W2036502140 title "Determination of the osmotic potential for swelling of cat brain in vitro" @default.
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- W2036502140 doi "https://doi.org/10.1016/0014-4886(79)90248-6" @default.
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