FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2036502435> ?p ?o ?g. }

• W2036502435 endingPage "34" @default.
• W2036502435 startingPage "26" @default.
• W2036502435 abstract "Several approaches to reduce acetate accumulation inEscherichia colicultures have recently been reported. This reduction subsequently led to a significant enhancement in recombinant protein production. In those studies, metabolically engineeredE. colistrains with reduced acetate synthesis rates were constructed through the modification of glucose uptake rate, the elimination of critical enzymes that are involved in the acetate formation pathways, and the redirection of carbon flux toward less inhibitory byproducts. In particular, it has been shown that strains carrying theBacillus subtilisacetolactate synthase (ALS) gene not only produce less acetate but also have a higher ATP yield. Metabolic flux analysis of carbon flux distribution of the central metabolic pathways and at the pyruvate branch point revealed that this strain has the ability to channel excess pyruvate to the much less toxic compound, acetoin. The main focus of this study is the systematic analysis of the effects of small perturbations in the host's existing pathways on the redistribution of carbon fluxes. Specifically, a mutant with deleted acetate kinase (ACK) and acetyl phosphotransferase (PTA) was constructed and studied. Results from the metabolic analysis of carbon redistribution show theackA-ptamutation will reduce acetate level at the expense of the growth rate. In addition, in theackA-ptadeficient strain a much higher lactate formation rate with simultaneously lower formate and ethanol synthesis rates was found. Expression of theB. subtilisALS inackA-ptamutants further reduces acetate levels while cell density similar to that of the parent strain is attained." @default.
• W2036502435 created "2016-06-24" @default.
• W2036502435 creator A5018102237 @default.
• W2036502435 creator A5039193081 @default.
• W2036502435 creator A5060387277 @default.
• W2036502435 creator A5078194128 @default.
• W2036502435 date "1999-01-01" @default.
• W2036502435 modified "2023-09-29" @default.
• W2036502435 title "Metabolic Flux Analysis ofEscherichia coliDeficient in the Acetate Production Pathway and Expressing theBacillus subtilisAcetolactate Synthase" @default.
• W2036502435 cites W1492021547 @default.
• W2036502435 cites W1496569297 @default.
• W2036502435 cites W1548158456 @default.
• W2036502435 cites W1558751910 @default.
• W2036502435 cites W1583901171 @default.
• W2036502435 cites W1592718804 @default.
• W2036502435 cites W1613092247 @default.
• W2036502435 cites W1696110758 @default.
• W2036502435 cites W1800773337 @default.
• W2036502435 cites W1804584144 @default.
• W2036502435 cites W1900222282 @default.
• W2036502435 cites W1913495312 @default.
• W2036502435 cites W1934575827 @default.
• W2036502435 cites W1938326461 @default.
• W2036502435 cites W1970928098 @default.
• W2036502435 cites W1990766540 @default.
• W2036502435 cites W2006107682 @default.
• W2036502435 cites W2026540766 @default.
• W2036502435 cites W2030409730 @default.
• W2036502435 cites W2034748505 @default.
• W2036502435 cites W2049167322 @default.
• W2036502435 cites W2054371965 @default.
• W2036502435 cites W2058429937 @default.
• W2036502435 cites W2101752169 @default.
• W2036502435 cites W2110039658 @default.
• W2036502435 cites W2112285562 @default.
• W2036502435 cites W2113394693 @default.
• W2036502435 cites W2129128768 @default.
• W2036502435 cites W2141653906 @default.
• W2036502435 cites W2150207908 @default.
• W2036502435 doi "https://doi.org/10.1006/mben.1998.0103" @default.
• W2036502435 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10935752" @default.
• W2036502435 hasPublicationYear "1999" @default.
• W2036502435 type Work @default.
• W2036502435 sameAs 2036502435 @default.
• W2036502435 citedByCount "77" @default.
• W2036502435 countsByYear W20365024352012 @default.
• W2036502435 countsByYear W20365024352013 @default.
• W2036502435 countsByYear W20365024352014 @default.
• W2036502435 countsByYear W20365024352015 @default.
• W2036502435 countsByYear W20365024352016 @default.
• W2036502435 countsByYear W20365024352017 @default.
• W2036502435 countsByYear W20365024352018 @default.
• W2036502435 countsByYear W20365024352019 @default.
• W2036502435 countsByYear W20365024352020 @default.
• W2036502435 countsByYear W20365024352021 @default.
• W2036502435 countsByYear W20365024352023 @default.
• W2036502435 crossrefType "journal-article" @default.
• W2036502435 hasAuthorship W2036502435A5018102237 @default.
• W2036502435 hasAuthorship W2036502435A5039193081 @default.
• W2036502435 hasAuthorship W2036502435A5060387277 @default.
• W2036502435 hasAuthorship W2036502435A5078194128 @default.
• W2036502435 hasConcept C100544194 @default.
• W2036502435 hasConcept C104317684 @default.
• W2036502435 hasConcept C111133373 @default.
• W2036502435 hasConcept C160941953 @default.
• W2036502435 hasConcept C161790260 @default.
• W2036502435 hasConcept C165135838 @default.
• W2036502435 hasConcept C178790620 @default.
• W2036502435 hasConcept C181199279 @default.
• W2036502435 hasConcept C185592680 @default.
• W2036502435 hasConcept C192989942 @default.
• W2036502435 hasConcept C20251656 @default.
• W2036502435 hasConcept C2777504003 @default.
• W2036502435 hasConcept C2777937268 @default.
• W2036502435 hasConcept C2778470496 @default.
• W2036502435 hasConcept C2779826215 @default.
• W2036502435 hasConcept C2780178953 @default.
• W2036502435 hasConcept C2780854706 @default.
• W2036502435 hasConcept C547475151 @default.
• W2036502435 hasConcept C55493867 @default.
• W2036502435 hasConcept C62231903 @default.
• W2036502435 hasConcept C68709404 @default.
• W2036502435 hasConceptScore W2036502435C100544194 @default.
• W2036502435 hasConceptScore W2036502435C104317684 @default.
• W2036502435 hasConceptScore W2036502435C111133373 @default.
• W2036502435 hasConceptScore W2036502435C160941953 @default.
• W2036502435 hasConceptScore W2036502435C161790260 @default.
• W2036502435 hasConceptScore W2036502435C165135838 @default.
• W2036502435 hasConceptScore W2036502435C178790620 @default.
• W2036502435 hasConceptScore W2036502435C181199279 @default.
• W2036502435 hasConceptScore W2036502435C185592680 @default.
• W2036502435 hasConceptScore W2036502435C192989942 @default.
• W2036502435 hasConceptScore W2036502435C20251656 @default.
• W2036502435 hasConceptScore W2036502435C2777504003 @default.
• W2036502435 hasConceptScore W2036502435C2777937268 @default.
• W2036502435 hasConceptScore W2036502435C2778470496 @default.
• W2036502435 hasConceptScore W2036502435C2779826215 @default.
• W2036502435 hasConceptScore W2036502435C2780178953 @default.

• next