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- W2036519962 abstract "A capillary electrophoresis (CE) method based on an enzymatic cycling reaction is developed to determine both NAD+ and NADH in a single cell in a single run. The detection limit can reach down to 0.2 amol of NAD+ and 1 amol of NADH with a homemade capillary electrophoresis laser-induced fluorescence (CE-LIF) setup. This method shows good reproducibility and specificity. After an intact cell is injected into the capillary and lysed using a Tesla coil, intracellular NAD+ and NADH were separated, incubated with the cycling buffer, and quantified by recording the amount of fluorescent product generated. Cellular NAD+ and NADH levels of a rat myoblast cell line were determined using this method. Both NAD+ and NADH levels decreased when the cells were exposed to oxidative stress induced by H2O2. This may be due to the activation of the DNA repair enzyme, poly(ADP−ribose) polymerase, in response to the oxidative damage imposed on DNA, since pretreatment of the cells with an inhibitor of these enzymes prevented the reduction of cellular NAD+ and NADH levels." @default.
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- W2036519962 date "2009-01-06" @default.
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- W2036519962 title "Determination of NAD<sup>+</sup> and NADH in a Single Cell under Hydrogen Peroxide Stress by Capillary Electrophoresis" @default.
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- W2036519962 doi "https://doi.org/10.1021/ac802249m" @default.
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