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• W2036529357 abstract "The chromosome number of Bryum criberrimum (n = 10) is reported for the first time and Barbula unguiculata (n = 14 + 2m) is the first North American report. Dicranum sabuletorum (n = 12), Leskea gracilescens (n = 11), and L. obscura (n = 11) are reported for the second time. Corroborative counts are given for Weissia controversa (n = 13), Grimmia apocarpa (n = 14), and Mnium cuspidatum (n = 12). Counts for Amblystegium juratzkanum (n = 20) and Atrichum angustatum (n = 8) differ from most other reports for these species. There have been few chromosome studies of mosses in the midwestern United States, and the Great Plains region has up to now been completely ignored. Because of its rather well studied moss flora (Conard, 1956), Iowa is a convenient midwestern state in which to initiate such studies. This paper reports on ten species collected in central and south central Iowa. MATERIALS AND METHODS Collections were made during the summer of 1966, the fall and winter of 1967, and the spring of 1968. Sporophytes approximately at meiosis were fixed immediately; gametophytes bearing immature sporophytes were allowed to develop further in plastic boxes containing vermiculite, maintained in a growth chamber at 180C, with a 13-hour photoperiod of 600 foot-candles illumination. After comparing Farmer's fluid and Newcomer's solution, we concluded that the latter fixative was entirely satisfactory. Capsules were fixed in Newcomer's solution for several hours, which cleared the walls, allowing inspection of the internal condition of a large number of capsules. When sporogenous tissue within the capsule had a certain white opacity when viewed by transmitted light, meiotic stages could usually be found. Such capsules were then removed and dissected in a drop of 45% acetic acid, which softened and prepared them for staining in saturated aceto-orcein in 45% acetic acid. Repeated squashing and staining was often necessary to achieve the proper stain intensity. Slides were placed on a warming plate at 600C to hasten staining. Once the desired intensity was reached, the saturated aceto-orcein was replaced with a solution of saturated aceto-orcein diluted by an equal amount of 45% acetic acid. The slides were then ringed with rubber cement and could be kept in good condition under refrigeration for up to four weeks. To make slides permanent, we simply removed the cover glass, gently washed the material on both cover glass and slide in 70% alcohol followed by absolute alcohol, then mounted in Diaphane. Voucher specimens were deposited in herbaria at Iowa State University, University of 1 This work was begun in the summer of 1966 at Iowa State University while the first author was on a College Teacher Research Participation project and continued in 1967-68 during an Academic Year Extension grant. Grateful acknowledgement is made to the National Science Foundation for its support in these programs. We thank Dr. A. J. Sharp for checking our identifications of specimens. 2 Life Sciences Division, Grays Harbor College, Aberdeen, Washington 98520. S Department of Botany and Plant Pathology, Iowa State University, Ames, Iowa 50010. This content downloaded from 207.46.13.90 on Thu, 15 Sep 2016 04:02:38 UTC All use subject to http://about.jstor.org/terms 1968] MESSMER AND LERSTEN: CHROMOSOME STUDIES OF MOSSES FROM IOWA 349" @default.
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• W2036529357 date "1968-01-01" @default.
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• W2036529357 title "Chromosome Studies of Ten Species of Mosses from Iowa" @default.
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• W2036529357 doi "https://doi.org/10.1639/0007-2745(1968)71[348:csotso]2.0.co;2" @default.
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