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• W2036536086 startingPage "863" @default.
• W2036536086 abstract "A beacon aptamer-based biosensor for the detection of thrombin was developed using electrochemical transduction method. Gold surface was modified with a beacon aptamer covalently linked at 5′-terminus with a linker containing a primary aliphatic amine. Methylene blue (MB) was intercalated into the beacon sequence, and used as an electrochemical marker. When the beacon aptamer immobilized on gold surface encounters thrombin, the hairpin forming beacon aptamer is conformationally changed to release the intercalated MB, resulting a decrease in electrical current intensity in voltamogram. The peak signal of the MB is clearly decreased by the binding of thrombin onto the beacon aptamer. The linear range of the signal was observed between 0 and 50.8 nM of thrombin with 0.999 correlation factor. This method was able to linearly and selectively detect thrombin with a detection limit of 11 nM." @default.
• W2036536086 created "2016-06-24" @default.
• W2036536086 creator A5034518298 @default.
• W2036536086 creator A5052315121 @default.
• W2036536086 creator A5075275136 @default.
• W2036536086 date "2005-12-01" @default.
• W2036536086 modified "2023-10-18" @default.
• W2036536086 title "A novel electrochemical detection method for aptamer biosensors" @default.
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• W2036536086 doi "https://doi.org/10.1016/j.bios.2005.02.002" @default.
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