FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2036542304> ?p ?o ?g. }

• W2036542304 endingPage "434" @default.
• W2036542304 startingPage "425" @default.
• W2036542304 abstract "Microtubule-associated proteins (MAPs) from calf brain were fluorescently labeled with 6-iodoacetamido fluorescein (I-AF). The modified MAPs (especially enriched for MAP2) were fully active in promoting tubulin polymerization in vitro and readily associated with cytoplasmic filaments when microinjected into living cultured cells. Double-labeling experiments indicated that the microinjected AF-MAPs were incorporated predominantly, if not exclusively, into cytoplasmic microtubules in untreated cells or paracrystals induced within vinblastine-treated cells. Similar results were obtained with different cell types (neuronal, epithelial, and fibroblastic) of diverse origin (man, mouse, chicken, and rat kangaroo). Mobility measurements of the microinjected AF-MAPs using the method of fluorescence-photobleaching recovery (FPR) revealed two populations of AF-MAPs with distinct dynamic properties: One fraction represents the soluble pool of MAPs and is mobile with a diffusion coefficient of D = 3 X 10(-9) cm2/s. The other fraction of MAPs is associated with the microtubules and is essentially immobile on the time scale of FPR experiments. However, it showed slow fluorescence recovery with an apparent half time of approximately 5 min. The slow recovery of fluorescence on defined photobleached microtubules occurred most probably by the incorporation of AF-MAPs from the soluble cytoplasmic pool into the bleached area. The bleached spot on defined microtubules remained essentially immobile during the slow recovery phase. These results suggest that MAPs can associate in vivo with microtubules of diverse cell types and that treadmilling of MAP2-containing microtubules in vivo, if it exists, is slower than 4 micron/h." @default.
• W2036542304 created "2016-06-24" @default.
• W2036542304 creator A5002316771 @default.
• W2036542304 creator A5046264807 @default.
• W2036542304 creator A5053719429 @default.
• W2036542304 creator A5067994300 @default.
• W2036542304 creator A5071701842 @default.
• W2036542304 creator A5088688022 @default.
• W2036542304 date "1984-08-01" @default.
• W2036542304 modified "2023-10-15" @default.
• W2036542304 title "Dynamic interactions of fluorescently labeled microtubule-associated proteins in living cells." @default.
• W2036542304 cites W1549481657 @default.
• W2036542304 cites W1558443685 @default.
• W2036542304 cites W1560764577 @default.
• W2036542304 cites W1606524691 @default.
• W2036542304 cites W1965137443 @default.
• W2036542304 cites W1974340985 @default.
• W2036542304 cites W1975040221 @default.
• W2036542304 cites W1975324638 @default.
• W2036542304 cites W1981204109 @default.
• W2036542304 cites W1984353845 @default.
• W2036542304 cites W1984675968 @default.
• W2036542304 cites W1986914550 @default.
• W2036542304 cites W1995562130 @default.
• W2036542304 cites W1995731871 @default.
• W2036542304 cites W2003468593 @default.
• W2036542304 cites W2007785335 @default.
• W2036542304 cites W2011521398 @default.
• W2036542304 cites W2012116143 @default.
• W2036542304 cites W2012580097 @default.
• W2036542304 cites W2013568696 @default.
• W2036542304 cites W2014636427 @default.
• W2036542304 cites W2014818256 @default.
• W2036542304 cites W2017903154 @default.
• W2036542304 cites W2017934591 @default.
• W2036542304 cites W2018289835 @default.
• W2036542304 cites W2030456302 @default.
• W2036542304 cites W2030548078 @default.
• W2036542304 cites W2034677151 @default.
• W2036542304 cites W2039303292 @default.
• W2036542304 cites W2051400138 @default.
• W2036542304 cites W2053914489 @default.
• W2036542304 cites W2058265493 @default.
• W2036542304 cites W2060300831 @default.
• W2036542304 cites W2066908713 @default.
• W2036542304 cites W2070072197 @default.
• W2036542304 cites W2075893848 @default.
• W2036542304 cites W2100837269 @default.
• W2036542304 cites W2131796461 @default.
• W2036542304 cites W2135829848 @default.
• W2036542304 cites W2137756635 @default.
• W2036542304 cites W2143588558 @default.
• W2036542304 cites W2411052607 @default.
• W2036542304 cites W961332773 @default.
• W2036542304 doi "https://doi.org/10.1083/jcb.99.2.425" @default.
• W2036542304 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2113270" @default.
• W2036542304 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6547721" @default.
• W2036542304 hasPublicationYear "1984" @default.
• W2036542304 type Work @default.
• W2036542304 sameAs 2036542304 @default.
• W2036542304 citedByCount "46" @default.
• W2036542304 countsByYear W20365423042013 @default.
• W2036542304 crossrefType "journal-article" @default.
• W2036542304 hasAuthorship W2036542304A5002316771 @default.
• W2036542304 hasAuthorship W2036542304A5046264807 @default.
• W2036542304 hasAuthorship W2036542304A5053719429 @default.
• W2036542304 hasAuthorship W2036542304A5067994300 @default.
• W2036542304 hasAuthorship W2036542304A5071701842 @default.
• W2036542304 hasAuthorship W2036542304A5088688022 @default.
• W2036542304 hasBestOaLocation W20365423041 @default.
• W2036542304 hasConcept C121332964 @default.
• W2036542304 hasConcept C12554922 @default.
• W2036542304 hasConcept C142669718 @default.
• W2036542304 hasConcept C1491633281 @default.
• W2036542304 hasConcept C153911025 @default.
• W2036542304 hasConcept C169274487 @default.
• W2036542304 hasConcept C189040839 @default.
• W2036542304 hasConcept C190062978 @default.
• W2036542304 hasConcept C20418707 @default.
• W2036542304 hasConcept C205309164 @default.
• W2036542304 hasConcept C207001950 @default.
• W2036542304 hasConcept C26659971 @default.
• W2036542304 hasConcept C2778015030 @default.
• W2036542304 hasConcept C41625074 @default.
• W2036542304 hasConcept C54355233 @default.
• W2036542304 hasConcept C55493867 @default.
• W2036542304 hasConcept C61081601 @default.
• W2036542304 hasConcept C62520636 @default.
• W2036542304 hasConcept C84425145 @default.
• W2036542304 hasConcept C86803240 @default.
• W2036542304 hasConcept C91881484 @default.
• W2036542304 hasConcept C95444343 @default.
• W2036542304 hasConceptScore W2036542304C121332964 @default.
• W2036542304 hasConceptScore W2036542304C12554922 @default.
• W2036542304 hasConceptScore W2036542304C142669718 @default.
• W2036542304 hasConceptScore W2036542304C1491633281 @default.
• W2036542304 hasConceptScore W2036542304C153911025 @default.
• W2036542304 hasConceptScore W2036542304C169274487 @default.

• next