Matches in SemOpenAlex for { <https://semopenalex.org/work/W2036557527> ?p ?o ?g. }
- W2036557527 endingPage "736" @default.
- W2036557527 startingPage "724" @default.
- W2036557527 abstract "Ankyrins, the adapters of the spectrin skeleton, are involved in local accumulation and stabilization of integral proteins to the appropriate membrane domains. In striated muscle, tissue-dependent alternative splicing generates unique Ank3 gene products (ankyrins-G); they share the Obscurin/Titin-Binding-related Domain (OTBD), a muscle-specific insert of the C-terminal domain which is highly conserved among ankyrin genes, and binds obscurin and titin to Ank1 gene products. We previously proposed that OTBD sequences constitute a novel domain of protein-protein interactions which confers ankyrins with specific cellular functions in muscle. Here we searched for muscle proteins binding to ankyrin-G OTBD by yeast two hybrid assay, and we found plectin and filamin C, two organizing elements of the cytoskeleton with essential roles in myogenesis, muscle cell cytoarchitecture, and muscle disease. The three proteins coimmunoprecipitate from skeletal muscle extracts and colocalize at costameres in adult muscle fibers. During in vitro myogenesis, muscle ankyrins-G are first expressed in postmitotic myocytes undergoing fusion to myotubes. In western blots of subcellular fractions from C2C12 cells, the majority of muscle ankyrins-G appear associated with membrane compartments. Occasional but not extensive co-localization at nascent costameres suggested that ankyrin-G interactions with plectin and filamin C are not involved in costamere assembly; they would rather reinforce stability and/or modulate molecular interactions in sarcolemma microdomains by establishing novel links between muscle-specific ankyrins-G and the two costameric dystrophin-associated glycoprotein and integrin-based protein complexes. These results report the first protein-protein interactions involving the ankyrin-G OTBD domain and support the hypothesis that OTBD sequences confer ankyrins with a gain of function in vertebrates, bringing further consolidation and resilience of the linkage between sarcomeres and sarcolemma." @default.
- W2036557527 created "2016-06-24" @default.
- W2036557527 creator A5001789921 @default.
- W2036557527 creator A5049897792 @default.
- W2036557527 creator A5057922976 @default.
- W2036557527 date "2011-04-01" @default.
- W2036557527 modified "2023-10-14" @default.
- W2036557527 title "Novel interactions of ankyrins-G at the costameres: The muscle-specific Obscurin/Titin-Binding-related Domain (OTBD) binds plectin and filamin C" @default.
- W2036557527 cites W1483041084 @default.
- W2036557527 cites W1498647634 @default.
- W2036557527 cites W166819888 @default.
- W2036557527 cites W192451759 @default.
- W2036557527 cites W1985318253 @default.
- W2036557527 cites W1998813619 @default.
- W2036557527 cites W2003036450 @default.
- W2036557527 cites W2006062026 @default.
- W2036557527 cites W2007101462 @default.
- W2036557527 cites W2007340832 @default.
- W2036557527 cites W2008516669 @default.
- W2036557527 cites W2009933321 @default.
- W2036557527 cites W2023166695 @default.
- W2036557527 cites W2025910811 @default.
- W2036557527 cites W2030550346 @default.
- W2036557527 cites W2031186829 @default.
- W2036557527 cites W2036577601 @default.
- W2036557527 cites W2040210492 @default.
- W2036557527 cites W2040941311 @default.
- W2036557527 cites W2043466778 @default.
- W2036557527 cites W2048908753 @default.
- W2036557527 cites W2051543771 @default.
- W2036557527 cites W2052269737 @default.
- W2036557527 cites W2055694197 @default.
- W2036557527 cites W2058035176 @default.
- W2036557527 cites W2061248737 @default.
- W2036557527 cites W2063707906 @default.
- W2036557527 cites W2068659250 @default.
- W2036557527 cites W2068754251 @default.
- W2036557527 cites W2091950241 @default.
- W2036557527 cites W2093693349 @default.
- W2036557527 cites W2099388247 @default.
- W2036557527 cites W2107575131 @default.
- W2036557527 cites W2108376995 @default.
- W2036557527 cites W2117731764 @default.
- W2036557527 cites W2118789629 @default.
- W2036557527 cites W2120618651 @default.
- W2036557527 cites W2133548874 @default.
- W2036557527 cites W2136444406 @default.
- W2036557527 cites W2138170368 @default.
- W2036557527 cites W2138553198 @default.
- W2036557527 cites W2158714788 @default.
- W2036557527 cites W2161492700 @default.
- W2036557527 cites W2161585901 @default.
- W2036557527 cites W2162589243 @default.
- W2036557527 cites W2163352063 @default.
- W2036557527 cites W2164713642 @default.
- W2036557527 cites W2167969614 @default.
- W2036557527 cites W2168322305 @default.
- W2036557527 cites W2171448616 @default.
- W2036557527 cites W222481110 @default.
- W2036557527 cites W4232086556 @default.
- W2036557527 doi "https://doi.org/10.1016/j.yexcr.2011.01.002" @default.
- W2036557527 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21223964" @default.
- W2036557527 hasPublicationYear "2011" @default.
- W2036557527 type Work @default.
- W2036557527 sameAs 2036557527 @default.
- W2036557527 citedByCount "24" @default.
- W2036557527 countsByYear W20365575272012 @default.
- W2036557527 countsByYear W20365575272013 @default.
- W2036557527 countsByYear W20365575272014 @default.
- W2036557527 countsByYear W20365575272015 @default.
- W2036557527 countsByYear W20365575272017 @default.
- W2036557527 countsByYear W20365575272018 @default.
- W2036557527 countsByYear W20365575272019 @default.
- W2036557527 countsByYear W20365575272020 @default.
- W2036557527 countsByYear W20365575272021 @default.
- W2036557527 countsByYear W20365575272022 @default.
- W2036557527 countsByYear W20365575272023 @default.
- W2036557527 crossrefType "journal-article" @default.
- W2036557527 hasAuthorship W2036557527A5001789921 @default.
- W2036557527 hasAuthorship W2036557527A5049897792 @default.
- W2036557527 hasAuthorship W2036557527A5057922976 @default.
- W2036557527 hasConcept C104317684 @default.
- W2036557527 hasConcept C105702510 @default.
- W2036557527 hasConcept C125705527 @default.
- W2036557527 hasConcept C142669718 @default.
- W2036557527 hasConcept C1491633281 @default.
- W2036557527 hasConcept C153911025 @default.
- W2036557527 hasConcept C182800266 @default.
- W2036557527 hasConcept C20563397 @default.
- W2036557527 hasConcept C207200792 @default.
- W2036557527 hasConcept C2776666470 @default.
- W2036557527 hasConcept C2779959927 @default.
- W2036557527 hasConcept C2780394045 @default.
- W2036557527 hasConcept C37140074 @default.
- W2036557527 hasConcept C3763915 @default.
- W2036557527 hasConcept C50244902 @default.
- W2036557527 hasConcept C55493867 @default.
- W2036557527 hasConcept C68731436 @default.