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• W2036574626 endingPage "13046" @default.
• W2036574626 startingPage "13029" @default.
• W2036574626 abstract "ABSTRACT Influenza A virus (IAV) uses the low pH in late endocytic vacuoles as a cue for penetration by membrane fusion. Here, we analyzed the prefusion reactions that prepare the core for uncoating after it has been delivered to the cytosol. We found that this priming process occurs in two steps that are mediated by the envelope-embedded M2 ion channel. The first weakens the interactions between the matrix protein, M1, and the viral ribonucleoprotein bundle. It involves a conformational change in a linker sequence and the C-terminal domain of M1 after exposure to a pH below 6.5. The second step is triggered by a pH of <6.0 and by the influx of K + ions. It causes additional changes in M1 as well as a loss of stability in the viral ribonucleoprotein bundle. Our results indicate that both the switch from Na + to K + in maturing endosomes and the decreasing pH are needed to prime IAV cores for efficient uncoating and infection of the host cell. IMPORTANCE The entry of IAV involves several steps, including endocytosis and fusion at late endosomes. Entry also includes disassembly of the viral core, which is composed of the viral ribonucleoproteins and the RNA genome. We have found that the uncoating process of IAV is initiated long before the core is delivered into the cytosol. M2, an ion channel in the viral membrane, is activated when the virus passes through early endosomes. Here, we show that protons entering the virus through M2 cause a conformational change in the matrix protein, M1. This weakens interactions between M1 and the viral ribonucleoproteins. A second change was found to occur when the virus enters late endosomes. The preacidified core is then exposed to a high concentration of K + , which affects the interactions between the ribonucleoproteins. Thus, when cores are finally delivered to the cytosol, they are already partially destabilized and, therefore, uncoating competent and infectious." @default.
• W2036574626 created "2016-06-24" @default.
• W2036574626 creator A5029808315 @default.
• W2036574626 creator A5030712570 @default.
• W2036574626 creator A5033110590 @default.
• W2036574626 creator A5073719214 @default.
• W2036574626 creator A5084914720 @default.
• W2036574626 creator A5086099466 @default.
• W2036574626 date "2014-11-15" @default.
• W2036574626 modified "2023-10-12" @default.
• W2036574626 title "Stepwise Priming by Acidic pH and a High K ⁺ Concentration Is Required for Efficient Uncoating of Influenza A Virus Cores after Penetration" @default.
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