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- W2036624858 abstract "SUMMARY With a view to developing putatively neutral markers based on Single Nucleotide Polymorphisms (SNPs) in the human malaria parasite, Plasmodium vivax , we utilized the published whole genome sequence information of P. falciparum and P. vivax to find a ~200 kb conserved syntenic region between these two species. We have selected 27 non-coding DNA fragments (in introns and intergenic regions) of variable length (300–750 bp) in P. vivax in this syntenic region. PCR of P. vivax isolates of a population sample from India could successfully amplify 17 fragments. Subsequently, DNA sequencing and sequence analysis confirmed the polymorphic status of only 11 fragments. Altogether, 18 SNPs were detected and 2 different measures of nucleotide diversity showed variable patterns across different fragments; in general, introns were less variable than the intergenic regions. All 11 polymorphic fragments were found to be evolving according to a neutral equilibrium model and thus could be utilized as putatively neutral markers for population genetic studies in P. vivax . Different molecular population genetics parameters were also estimated, providing initial insight into the population genetics of Indian P. vivax ." @default.
- W2036624858 created "2016-06-24" @default.
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- W2036624858 date "2010-07-01" @default.
- W2036624858 modified "2023-09-27" @default.
- W2036624858 title "Single nucleotide polymorphisms, putatively neutral DNA markers and population genetic parameters in Indian Plasmodium vivax isolates" @default.
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- W2036624858 doi "https://doi.org/10.1017/s0031182010000533" @default.
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