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• W2036638991 abstract "Time-integrated and time-resolved fluorescence spectroscopies have been used to probe the photophysical properties of ethidium bromide (Eb) complexed to calf thymus DNA (ct-DNA). Fluorescence decay profiles are obtained using the technique of time-correlated single photon counting (TCSPC), and subsequently analysed using conventional sum-of-exponential (SOE) routines and also the maximum entropy method (MEM). Through use of these methods and simulated decay data, it is demonstrated that the kinetics of Eb in the presence of ds-DNA are best described by a generic model consisting of three exponential terms. At all DNA:Eb ratios and NaCl concentrations studied, free Eb is detected. Furthermore, Eb is found to interact with ds-DNA through two mechanisms, each distinguishable by its fluorescence decaytime. Eb is shown to interact with DNA through classic intercalation, and also through binding at secondary sites. The component decaytimes are shown to be a function of NaCl concentration but independent of DNA:Eb molar ratio." @default.
• W2036638991 created "2016-06-24" @default.
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• W2036638991 creator A5060939871 @default.
• W2036638991 date "1998-03-01" @default.
• W2036638991 modified "2023-10-10" @default.
• W2036638991 title "Photophysics of ethidium bromide complexed to ct-DNA: a maximum entropy study" @default.
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• W2036638991 doi "https://doi.org/10.1016/s0301-4622(97)00091-4" @default.
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