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• W2036680775 abstract "RNA sequences near poly(A) of adenovirus-2 late messenger RNAs have been mapped within the Ad2 genome and compared by T1 ribonuclease fingerprinting. Polyadenylated viral mRNAs transcribed 14 to 17 hours post-infection were prepared highly labeled with 32P, and then fractionated by hybridization to restriction fragments of Ad2 DNA or by gel electrophoresis. The 3′-terminal regions of purified mRNAs were isolated by partially degrading the mRNA with alkali or T1 ribonuclease to an average length of ~100 to 200 residues, and selecting fragments with poly(A) by poly(U)-Sepharose chromatography. Six different 3′-terminal structures, all derived from rightward transcripts, were identified. The map positions of these structures were initially deduced by establishing the fingerprints of sequences near poly(A) of mRNAs hybridized to DNA restriction fragments with known map co-ordinates. In four cases the DNA templates for the termini were located more precisely by identifying short restriction fragments of known map position which encode, in vitro, large T1 oligo-nucleotides found in vivo near poly(A). Five 3′ termini map within restriction fragments Sma1 I/HinIII J (co-ordinates 37.3 to 40.5), Sma1 D/Kpn D (47.4 to 52.6), EcoRI B (58.5 to 70.7), EcoRI D (75.9 to 83.4), and Sma1 C/Eco R1 C (89.7 to 91.9). These 3′ termini fall within the boundaries of the major Ad2 late transcription unit (co-ordinates ~16 to 100; Evans et al., 1977). The sixth terminus maps outside this region in Sma1 M (11.2 to 11.6) and thus is transcribed from a second transcription unit which also functions late in infection. Each of the six 3′ termini yielded large T1 oligonucleotides which were specific to the particular 3′-terminal region, rather than common to different structures. The hexanucleotide sequence A-A-U-A-A-A, which is located near poly(A) of several previously studied eukaryotic messengers (Proudfoot & Brownlee, 1976), is present in at least two of the Ad2 3′-terminal structures, and potentially in all six. The RNA sequences surrounding the hexanucleotide are not identical in the different viral cases, although these mRNAs are transcribed from a single DNA genome. The location of Ad2 mRNA 3′-terminal sequences within the viral genome is discussed in terms of the Ad2 late mRNA map and a hypothesis for the role of 3′ terminus formation in Ad2 mRNA synthesis." @default.
• W2036680775 created "2016-06-24" @default.
• W2036680775 creator A5057971828 @default.
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• W2036680775 date "1978-09-01" @default.
• W2036680775 modified "2023-09-23" @default.
• W2036680775 title "RNA structures near poly(A) of adenovirus-2 late messenger RNAs" @default.
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• W2036680775 doi "https://doi.org/10.1016/0022-2836(78)90145-6" @default.
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• W2036680775 hasPublicationYear "1978" @default.
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