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- W2036701598 abstract "The activity of calf thymus DNA ligase was stimulated 2- to 3-fold by the addition of putrescine, spermidine and spermine. The optimal concentrations were 5 mM for putrescine, 0.5 mM for spermidine and 0.05–0.1 mM for spermine. Spermidine not only lowered the apparent Km value for Mg2+ but also elevated the V value, whereas the apparent Km value for ATP was not changed. In the presence of Mn2+ as a divalent cation, the V value was stimulated 3-fold by 0.5 mM spermidine. Monovalent cations, such as KCl and NaCl could replace the polyamines at the optimal concentrations of 50 mM and diminish the stimulation by the polyamines." @default.
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- W2036701598 title "Activation of mammalian DNA ligase by polyamines" @default.
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- W2036701598 doi "https://doi.org/10.1016/0006-291x(80)90833-5" @default.
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