Matches in SemOpenAlex for { <https://semopenalex.org/work/W2036703182> ?p ?o ?g. }
- W2036703182 endingPage "1270" @default.
- W2036703182 startingPage "1261" @default.
- W2036703182 abstract "Intestinal disorders such as inflammatory bowel disease (IBD) result in chronic illness requiring lifelong therapy. Our aim was to evaluate the efficacy of recombinant adeno-associated virus (AAV) vector-mediated gene delivery to intestinal epithelial cells in vitro and in vivo. Human colon epithelial cell lines and colon biopsies were transduced using AAV pseudotypes 2/1, 2/2, and 2/5 encoding green fluorescence protein (GFP). Mice were administered the same vectors through oral, enema, intraperitoneal (IP) injection and superior mesenteric artery (SMA) injection routes. Tropism and efficiency were determined by microscopy, flow cytometry, immunohistochemistry and PCR. Caco2 cells were more permissive to AAV transduction. Human colon epithelial cells in organ culture were more effectively transduced by AAV2/2. SMA injection provided the most effective means of vector gene transfer to small intestine and colonic epithelial cells in vivo. Transgene detection 80 days post AAV treatment suggests transduction of crypt progenitor cells. This study shows the feasibility of AAV-mediated intestinal gene delivery, applicable for the investigation of IBD pathogenesis and novel therapeutic options, but also revealed the need for further studies to identify more efficient pseudotypes." @default.
- W2036703182 created "2016-06-24" @default.
- W2036703182 creator A5009567375 @default.
- W2036703182 creator A5023903232 @default.
- W2036703182 creator A5053845478 @default.
- W2036703182 creator A5062727443 @default.
- W2036703182 creator A5064422415 @default.
- W2036703182 creator A5083246410 @default.
- W2036703182 creator A5090006240 @default.
- W2036703182 date "2007-10-13" @default.
- W2036703182 modified "2023-10-18" @default.
- W2036703182 title "Gene Delivery to Intestinal Epithelial Cells In vitro and In vivo with Recombinant Adeno-Associated Virus Types 1, 2 and 5" @default.
- W2036703182 cites W1490724066 @default.
- W2036703182 cites W1529885495 @default.
- W2036703182 cites W1654216462 @default.
- W2036703182 cites W1968382713 @default.
- W2036703182 cites W1973897072 @default.
- W2036703182 cites W1975528602 @default.
- W2036703182 cites W1995691954 @default.
- W2036703182 cites W2000191112 @default.
- W2036703182 cites W2002134849 @default.
- W2036703182 cites W2006072627 @default.
- W2036703182 cites W2008254103 @default.
- W2036703182 cites W2014010760 @default.
- W2036703182 cites W2025185410 @default.
- W2036703182 cites W2026539870 @default.
- W2036703182 cites W2035337429 @default.
- W2036703182 cites W2036042297 @default.
- W2036703182 cites W2045415534 @default.
- W2036703182 cites W2047608515 @default.
- W2036703182 cites W2048008983 @default.
- W2036703182 cites W2050821526 @default.
- W2036703182 cites W2051159468 @default.
- W2036703182 cites W2060637142 @default.
- W2036703182 cites W2061494785 @default.
- W2036703182 cites W2066278712 @default.
- W2036703182 cites W2081064825 @default.
- W2036703182 cites W2085577956 @default.
- W2036703182 cites W2086436015 @default.
- W2036703182 cites W2100394645 @default.
- W2036703182 cites W2113259183 @default.
- W2036703182 cites W2133458483 @default.
- W2036703182 cites W2143864438 @default.
- W2036703182 cites W2154329712 @default.
- W2036703182 cites W2165815741 @default.
- W2036703182 cites W2177438840 @default.
- W2036703182 cites W227138407 @default.
- W2036703182 cites W2321201401 @default.
- W2036703182 cites W4232023110 @default.
- W2036703182 cites W4313379449 @default.
- W2036703182 cites W45551491 @default.
- W2036703182 cites W2070768477 @default.
- W2036703182 doi "https://doi.org/10.1007/s10620-007-9991-1" @default.
- W2036703182 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3896329" @default.
- W2036703182 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17934813" @default.
- W2036703182 hasPublicationYear "2007" @default.
- W2036703182 type Work @default.
- W2036703182 sameAs 2036703182 @default.
- W2036703182 citedByCount "32" @default.
- W2036703182 countsByYear W20367031822012 @default.
- W2036703182 countsByYear W20367031822013 @default.
- W2036703182 countsByYear W20367031822015 @default.
- W2036703182 countsByYear W20367031822016 @default.
- W2036703182 countsByYear W20367031822020 @default.
- W2036703182 countsByYear W20367031822021 @default.
- W2036703182 countsByYear W20367031822022 @default.
- W2036703182 countsByYear W20367031822023 @default.
- W2036703182 crossrefType "journal-article" @default.
- W2036703182 hasAuthorship W2036703182A5009567375 @default.
- W2036703182 hasAuthorship W2036703182A5023903232 @default.
- W2036703182 hasAuthorship W2036703182A5053845478 @default.
- W2036703182 hasAuthorship W2036703182A5062727443 @default.
- W2036703182 hasAuthorship W2036703182A5064422415 @default.
- W2036703182 hasAuthorship W2036703182A5083246410 @default.
- W2036703182 hasAuthorship W2036703182A5090006240 @default.
- W2036703182 hasBestOaLocation W20367031822 @default.
- W2036703182 hasConcept C104317684 @default.
- W2036703182 hasConcept C111599444 @default.
- W2036703182 hasConcept C135983454 @default.
- W2036703182 hasConcept C142613039 @default.
- W2036703182 hasConcept C142724271 @default.
- W2036703182 hasConcept C150903083 @default.
- W2036703182 hasConcept C15152581 @default.
- W2036703182 hasConcept C153911025 @default.
- W2036703182 hasConcept C159047783 @default.
- W2036703182 hasConcept C207001950 @default.
- W2036703182 hasConcept C2522874641 @default.
- W2036703182 hasConcept C2778107364 @default.
- W2036703182 hasConcept C2778260677 @default.
- W2036703182 hasConcept C2779134260 @default.
- W2036703182 hasConcept C32470452 @default.
- W2036703182 hasConcept C35693153 @default.
- W2036703182 hasConcept C40767141 @default.
- W2036703182 hasConcept C55493867 @default.
- W2036703182 hasConcept C71924100 @default.
- W2036703182 hasConcept C86803240 @default.
- W2036703182 hasConcept C92087593 @default.
- W2036703182 hasConceptScore W2036703182C104317684 @default.