Matches in SemOpenAlex for { <https://semopenalex.org/work/W2036703358> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W2036703358 endingPage "141" @default.
- W2036703358 startingPage "136" @default.
- W2036703358 abstract "Preliminary work revealed that nitrate reductase in crude extracts prepared from leaves of certain corn genotypes as well as soybeans could utilize NADPH as well as NADH as the electron donor. Isoelectric focusing and diethylaminoethyl cellulose chromatography confirmed previous findings that NADH and NADPH activities could not be separated, which suggests the involvement of a single enzyme. Nitrate reduction with both cofactors varies with plant species, plant age, and assay conditions. The ability of the nitrate reductase from a given genotype to utilize NADPH was associated with the amount of NADPH-phosphatase in the extract. While diethylaminoethyl cellulose chromatography of plant extracts separated nitrate reductase from the bulk (90%) of the phosphatase and caused a decrease in the NADPH activity, the residual level of phosphatase was sufficient to account for the apparent NADPH nitrate reductase activity. Addition of KH(2)PO(4) and KF, inhibitors of NADPH-phosphatase activity in in vitro assays, caused a drastic reduction or abolishment of NADPH-mediated nitrate reductase activity but were without effect on NADH nitrate reductase activity. It is concluded that NADPH-nitrate reduction, in soybean and certain corn genotypes, is an artifact resulting from the conversion of NADPH to NADH by a phosphatase and that the enzyme in leaf tissue is NADH-dependent (E.C.1.6.6.1)." @default.
- W2036703358 created "2016-06-24" @default.
- W2036703358 creator A5017304753 @default.
- W2036703358 creator A5082442764 @default.
- W2036703358 date "1974-08-01" @default.
- W2036703358 modified "2023-10-16" @default.
- W2036703358 title "Specificity for Nicotinamide Adenine Dinucleotide by Nitrate Reductase from Leaves" @default.
- W2036703358 doi "https://doi.org/10.1104/pp.54.2.136" @default.
- W2036703358 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/541519" @default.
- W2036703358 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16658848" @default.
- W2036703358 hasPublicationYear "1974" @default.
- W2036703358 type Work @default.
- W2036703358 sameAs 2036703358 @default.
- W2036703358 citedByCount "35" @default.
- W2036703358 countsByYear W20367033582019 @default.
- W2036703358 crossrefType "journal-article" @default.
- W2036703358 hasAuthorship W2036703358A5017304753 @default.
- W2036703358 hasAuthorship W2036703358A5082442764 @default.
- W2036703358 hasBestOaLocation W20367033581 @default.
- W2036703358 hasConcept C134651460 @default.
- W2036703358 hasConcept C178790620 @default.
- W2036703358 hasConcept C181199279 @default.
- W2036703358 hasConcept C185592680 @default.
- W2036703358 hasConcept C197957613 @default.
- W2036703358 hasConcept C2776384668 @default.
- W2036703358 hasConcept C2777989768 @default.
- W2036703358 hasConcept C2778710519 @default.
- W2036703358 hasConcept C28212737 @default.
- W2036703358 hasConcept C38485361 @default.
- W2036703358 hasConcept C55493867 @default.
- W2036703358 hasConcept C75520062 @default.
- W2036703358 hasConceptScore W2036703358C134651460 @default.
- W2036703358 hasConceptScore W2036703358C178790620 @default.
- W2036703358 hasConceptScore W2036703358C181199279 @default.
- W2036703358 hasConceptScore W2036703358C185592680 @default.
- W2036703358 hasConceptScore W2036703358C197957613 @default.
- W2036703358 hasConceptScore W2036703358C2776384668 @default.
- W2036703358 hasConceptScore W2036703358C2777989768 @default.
- W2036703358 hasConceptScore W2036703358C2778710519 @default.
- W2036703358 hasConceptScore W2036703358C28212737 @default.
- W2036703358 hasConceptScore W2036703358C38485361 @default.
- W2036703358 hasConceptScore W2036703358C55493867 @default.
- W2036703358 hasConceptScore W2036703358C75520062 @default.
- W2036703358 hasIssue "2" @default.
- W2036703358 hasLocation W20367033581 @default.
- W2036703358 hasLocation W20367033582 @default.
- W2036703358 hasLocation W20367033583 @default.
- W2036703358 hasOpenAccess W2036703358 @default.
- W2036703358 hasPrimaryLocation W20367033581 @default.
- W2036703358 hasRelatedWork W2005913395 @default.
- W2036703358 hasRelatedWork W2049271139 @default.
- W2036703358 hasRelatedWork W2084964391 @default.
- W2036703358 hasRelatedWork W2114004664 @default.
- W2036703358 hasRelatedWork W2119073376 @default.
- W2036703358 hasRelatedWork W2137996948 @default.
- W2036703358 hasRelatedWork W2325540509 @default.
- W2036703358 hasRelatedWork W2596200008 @default.
- W2036703358 hasRelatedWork W294831262 @default.
- W2036703358 hasRelatedWork W2188842545 @default.
- W2036703358 hasVolume "54" @default.
- W2036703358 isParatext "false" @default.
- W2036703358 isRetracted "false" @default.
- W2036703358 magId "2036703358" @default.
- W2036703358 workType "article" @default.