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• W2036716863 abstract "Isolated transverse tubule vesicles free of sarcoplasmic reticulum transport calcium with high affinity in the presence of ATP. The calcium transport by transverse tubules differs from calcium transport by sarcoplasmic reticulum. It is not increased by oxalate or phosphate, it has a different temperature dependence, it is inhibited by sub-micromolar concentrations of orthovanadate, it is stimulated by calmodulin, and is inhibited by quercetin without causing calcium release. The rates of calcium transport by transverse tubules are two orders of magnitude lower than those of sarcoplasmic reticulum, suggesting that the calcium pump protein of transverse tubules is a minor component of the membrane. Addition of calmodulin to transverse tubule vesicles - treated with high salt in the presence of EGTA to remove endogenous calmodulin - caused a marked stimulation of transport rates at low concentrations of calcium, and decreased from 1.0 to 0.3 μM the calcium concentration at which half-maximal rates of transport were obtained. A role for the transverse tubule calcium pump in maintaining low sarcoplasmic calcium concentrations is proposed." @default.
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• W2036716863 date "1986-01-01" @default.
• W2036716863 modified "2023-10-15" @default.
• W2036716863 title "Calcium transport in transverse tubules isolated from rabbit skeletal muscle" @default.
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• W2036716863 doi "https://doi.org/10.1016/0005-2736(86)90121-5" @default.
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