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• W2036719012 abstract "Objectives: The purpose of this study was to investigate the differential expressions of human beta defensin ( hBD) 2 and hBD-3 in human middle ear cholesteatoma epithelium. Methods: The expressions of hBD-2 and hBD-3 were analyzed by quantitative real-time reverse transcription-poly-merase chain reaction (RT-PCR), Western blotting, and immunohistochemical staining. Samples were obtained from 10 patients who underwent middle ear surgery for middle ear cholesteatoma. Results: Real-time RT-PCR and Western blot analysis showed that the messenger RNAs and proteins of hBD-2 and hBD-3 were higher in the cholesteatoma epithelium than in normal external auditory canal skin. In cholesteatoma epithelium, hBD-2 and hBD-3 activities were present in the upper granular layer and in the prickle cell layer, but in the normal skin they were poorly expressed in all layers. Conclusions: Increased expressions of hBD-2 and hBD-3 in cholesteatoma epithelium suggest that cholesteatoma, a chronic inflammatory state of middle ear keratinocytes, may induce an innate immune response. That the induction of hBD-2 was found to be more intense than that of hBD-3 in cholesteatoma epithelium implies that hBD-2 is the major effector in terms of chronic epithelial inflammatory responses." @default.
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• W2036719012 date "2007-03-01" @default.
• W2036719012 modified "2023-10-16" @default.
• W2036719012 title "Differential Expression of Human Beta Defensin 2 and Human Beta Defensin 3 in Human Middle Ear Cholesteatoma" @default.
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• W2036719012 doi "https://doi.org/10.1177/000348940711600312" @default.
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