Matches in SemOpenAlex for { <https://semopenalex.org/work/W2036719651> ?p ?o ?g. }
- W2036719651 endingPage "1290" @default.
- W2036719651 startingPage "1277" @default.
- W2036719651 abstract "Bile acids play important physiological role in the solubilisation and absorption of dietary lipids. However, under pathophysiological conditions, such as short bowel syndrome, they can reach the colon in high concentrations inducing diarrhoea. In this study, our aim was to characterise the cellular pathomechanism of bile-induced diarrhoea using human samples. Colonic crypts were isolated from biopsies of patients (controls with negative colonoscopic findings) and of cholecystectomised/ileum-resected patients with or without diarrhoea. In vitro measurement of the transporter activities revealed impaired Na+/H+ exchanger (NHE) and Cl−/HCO3 − exchanger (CBE) activities in cholecystectomised/ileum-resected patients suffering from diarrhoea, compared to control patients. Acute treatment of colonic crypts with 0.3 mM chenodeoxycholate caused dose-dependent intracellular acidosis; moreover, the activities of acid/base transporters (NHE and CBE) were strongly impaired. This concentration of chenodeoxycholate did not cause morphological changes in colonic epithelial cells, although significantly reduced the intracellular ATP level, decreased mitochondrial transmembrane potential and caused sustained intracellular Ca2+ elevation. We also showed that chenodeoxycholate induced Ca2+ release from the endoplasmic reticulum and extracellular Ca2+ influx contributing to the Ca2+ elevation. Importantly, our results suggest that the chenodeoxycholate-induced inhibition of NHE activities was ATP-dependent, whereas the inhibition of CBE activity was mediated by the sustained Ca2+ elevation. We suggest that bile acids inhibit the function of ion transporters via cellular energy breakdown and Ca2+ overload in human colonic epithelial cells, which can reduce fluid and electrolyte absorption in the colon and promote the development of diarrhoea." @default.
- W2036719651 created "2016-06-24" @default.
- W2036719651 creator A5000144279 @default.
- W2036719651 creator A5017500029 @default.
- W2036719651 creator A5025810535 @default.
- W2036719651 creator A5042057933 @default.
- W2036719651 creator A5054997401 @default.
- W2036719651 creator A5063153069 @default.
- W2036719651 creator A5070315504 @default.
- W2036719651 creator A5071674782 @default.
- W2036719651 creator A5082236738 @default.
- W2036719651 creator A5085304203 @default.
- W2036719651 creator A5086657850 @default.
- W2036719651 creator A5090767185 @default.
- W2036719651 date "2014-07-13" @default.
- W2036719651 modified "2023-10-15" @default.
- W2036719651 title "Bile acids inhibit Na+/H+ exchanger and Cl−/HCO3 − exchanger activities via cellular energy breakdown and Ca2+ overload in human colonic crypts" @default.
- W2036719651 cites W1511903352 @default.
- W2036719651 cites W1529695053 @default.
- W2036719651 cites W1533528446 @default.
- W2036719651 cites W1589657989 @default.
- W2036719651 cites W1820315269 @default.
- W2036719651 cites W1974342707 @default.
- W2036719651 cites W1991438124 @default.
- W2036719651 cites W2008979188 @default.
- W2036719651 cites W2010792110 @default.
- W2036719651 cites W2017055754 @default.
- W2036719651 cites W2023271914 @default.
- W2036719651 cites W2026050056 @default.
- W2036719651 cites W2031989685 @default.
- W2036719651 cites W2032781929 @default.
- W2036719651 cites W2033936610 @default.
- W2036719651 cites W2039259374 @default.
- W2036719651 cites W2039952090 @default.
- W2036719651 cites W2052036406 @default.
- W2036719651 cites W2072584309 @default.
- W2036719651 cites W2076978195 @default.
- W2036719651 cites W2078492653 @default.
- W2036719651 cites W2082159651 @default.
- W2036719651 cites W2090351035 @default.
- W2036719651 cites W2098462724 @default.
- W2036719651 cites W2105431222 @default.
- W2036719651 cites W2107231357 @default.
- W2036719651 cites W2115217458 @default.
- W2036719651 cites W2122708050 @default.
- W2036719651 cites W2124723056 @default.
- W2036719651 cites W2130449935 @default.
- W2036719651 cites W2131300962 @default.
- W2036719651 cites W2131467340 @default.
- W2036719651 cites W2132663693 @default.
- W2036719651 cites W2133411368 @default.
- W2036719651 cites W2144302045 @default.
- W2036719651 cites W2144603992 @default.
- W2036719651 cites W2144658887 @default.
- W2036719651 cites W2153062701 @default.
- W2036719651 cites W2164824799 @default.
- W2036719651 cites W2165873335 @default.
- W2036719651 cites W2165877587 @default.
- W2036719651 cites W2169141456 @default.
- W2036719651 cites W2171493598 @default.
- W2036719651 doi "https://doi.org/10.1007/s00424-014-1560-9" @default.
- W2036719651 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25015415" @default.
- W2036719651 hasPublicationYear "2014" @default.
- W2036719651 type Work @default.
- W2036719651 sameAs 2036719651 @default.
- W2036719651 citedByCount "11" @default.
- W2036719651 countsByYear W20367196512015 @default.
- W2036719651 countsByYear W20367196512016 @default.
- W2036719651 countsByYear W20367196512018 @default.
- W2036719651 countsByYear W20367196512019 @default.
- W2036719651 countsByYear W20367196512022 @default.
- W2036719651 crossrefType "journal-article" @default.
- W2036719651 hasAuthorship W2036719651A5000144279 @default.
- W2036719651 hasAuthorship W2036719651A5017500029 @default.
- W2036719651 hasAuthorship W2036719651A5025810535 @default.
- W2036719651 hasAuthorship W2036719651A5042057933 @default.
- W2036719651 hasAuthorship W2036719651A5054997401 @default.
- W2036719651 hasAuthorship W2036719651A5063153069 @default.
- W2036719651 hasAuthorship W2036719651A5070315504 @default.
- W2036719651 hasAuthorship W2036719651A5071674782 @default.
- W2036719651 hasAuthorship W2036719651A5082236738 @default.
- W2036719651 hasAuthorship W2036719651A5085304203 @default.
- W2036719651 hasAuthorship W2036719651A5086657850 @default.
- W2036719651 hasAuthorship W2036719651A5090767185 @default.
- W2036719651 hasBestOaLocation W20367196512 @default.
- W2036719651 hasConcept C104317684 @default.
- W2036719651 hasConcept C114574056 @default.
- W2036719651 hasConcept C126322002 @default.
- W2036719651 hasConcept C134018914 @default.
- W2036719651 hasConcept C134897140 @default.
- W2036719651 hasConcept C149011108 @default.
- W2036719651 hasConcept C178790620 @default.
- W2036719651 hasConcept C185592680 @default.
- W2036719651 hasConcept C2777226302 @default.
- W2036719651 hasConcept C2779399885 @default.
- W2036719651 hasConcept C28406088 @default.
- W2036719651 hasConcept C2910800957 @default.
- W2036719651 hasConcept C537181965 @default.