FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2036725530> ?p ?o ?g. }

• W2036725530 endingPage "986" @default.
• W2036725530 startingPage "973" @default.
• W2036725530 abstract "Fluorescence imaging of indo-1 loaded cells was used to monitor influx and distribution of Ca2+ in cell bodies, neurites and growth cones of sympathetic neurons cultured from embryonic chick. Similar experiments on release of tritiated noradrenaline were performed to assess the relationship between intracellular Ca2+ concentration ([Ca2+]) and transmitter release. Effects of Ca2+ channel antagonists on electrically stimulated rise in [Ca2+]i were dependent on the neuronal region examined. Cadmium and verapamil blocked Ca2+ entry in cell bodies but were less effective in neurites and growth cones. Nifedipine partially inhibited Ca2+ entry in cell bodies and was less effective in neurites and growth cones. Combination of cadmium and nifedipine blocked [Ca2+]i rise in all neuronal regions. Omega-conotoxin was an effective Ca2+ channel blocker in all regions. Ca2+ channel blockers had effects on [3H]noradrenaline release which paralleled effects on [Ca2+]i in neurites (and growth cones) but not cell bodies. Cadmium, verapamil and nifedipine each caused a partial, reversible block of the evoked release. Combination of cadmium and nifedipine completely blocked evoked [3+H]noradrenaline release. Omegaconotoxin caused complete, irreversible block of electrically evoked release. During prolonged depolarization with 125 mM K+ Krebs solution, elevation of [Ca2+]i was maintained in cell bodies but was transient in neurites and growth cones. The amplitude and time course of [3H]noradrenaline release paralleled [Ca2+]i in neurites and growth cones, but not the cell body under the above conditions. A new method is described to study localized uptake and release of [3H]noradrenaline in cell bodies versus neurites of sympathetic neurons. Incubation of these modified cultures with [3H]noradrenaline showed that cell bodies had very low [3H]noradrenaline uptake (0.23 × 10−6 c.p.m./mg protein), whereas neurites contained approximately 20 times more radioactivity. Depolarization of neurites by excess K+ and field stimulation caused a large increase in the net release of [3H]noradrenaline. The release was unaffected by removal of cell bodies. Neurites remained functionally viable for more than 2 h after separation from their cell bodies. [3H]Noradrenaline release could be evoked repeatedly over this time. [3H]Noradrenaline release from isolated neurites was partially blocked by nifedipine and fully blocked by combination of cadmium and nifedipine or by omega-conotoxin. The uptake and release of [3H]noradrenaline by neurites alone (expressed per mg protein) accounted for the total [3H]noradrenaline in intact cultures with neurites and cell bodies. Therefore, we conclude that neurites (and growth cones) are the prominent sites of uptake, storage and release of sympathetic transmitter. The regulation of Ca2+ influx and distribution is distinctly different in different neuronal regions and [Ca2+], of neurites and growth cones only are related to [3H]noradrenaline release. Partial effects of cadmium or nifedipine indicate the involvement of more than one Ca2+ channel type in Ca2+ entry and [3H]noradrenaline release. Regional variation in the effects of these agents suggests regional differences in the types of Ca2+ channel. The complete block of [3H]noradrenaline release and Ca2+ entry in all regions by omega-conotoxin indicates effective block of all neuronal Ca2+ channel types." @default.
• W2036725530 created "2016-06-24" @default.
• W2036725530 creator A5010862203 @default.
• W2036725530 creator A5042264523 @default.
• W2036725530 creator A5057558331 @default.
• W2036725530 creator A5077467792 @default.
• W2036725530 creator A5078080372 @default.
• W2036725530 date "1993-02-01" @default.
• W2036725530 modified "2023-10-17" @default.
• W2036725530 title "Sites of transmitter release and relation to intracellular Ca2+ in cultured sympathetic neurons" @default.
• W2036725530 cites W1521357599 @default.
• W2036725530 cites W1625394695 @default.
• W2036725530 cites W1975691097 @default.
• W2036725530 cites W1976584995 @default.
• W2036725530 cites W1983423336 @default.
• W2036725530 cites W2012143436 @default.
• W2036725530 cites W2012828388 @default.
• W2036725530 cites W2016493331 @default.
• W2036725530 cites W2023638493 @default.
• W2036725530 cites W2026351583 @default.
• W2036725530 cites W2030740718 @default.
• W2036725530 cites W2031753737 @default.
• W2036725530 cites W2040214977 @default.
• W2036725530 cites W2052344768 @default.
• W2036725530 cites W2053321947 @default.
• W2036725530 cites W2053559739 @default.
• W2036725530 cites W2055954298 @default.
• W2036725530 cites W2061056399 @default.
• W2036725530 cites W2064360667 @default.
• W2036725530 cites W2066352663 @default.
• W2036725530 cites W2068617048 @default.
• W2036725530 cites W2074730021 @default.
• W2036725530 cites W2087215029 @default.
• W2036725530 cites W2110071591 @default.
• W2036725530 cites W2118309627 @default.
• W2036725530 cites W2124711960 @default.
• W2036725530 cites W2141260882 @default.
• W2036725530 cites W2154492664 @default.
• W2036725530 cites W2318256351 @default.
• W2036725530 cites W993271046 @default.
• W2036725530 doi "https://doi.org/10.1016/0306-4522(93)90544-p" @default.
• W2036725530 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8450982" @default.
• W2036725530 hasPublicationYear "1993" @default.
• W2036725530 type Work @default.
• W2036725530 sameAs 2036725530 @default.
• W2036725530 citedByCount "42" @default.
• W2036725530 countsByYear W20367255302014 @default.
• W2036725530 crossrefType "journal-article" @default.
• W2036725530 hasAuthorship W2036725530A5010862203 @default.
• W2036725530 hasAuthorship W2036725530A5042264523 @default.
• W2036725530 hasAuthorship W2036725530A5057558331 @default.
• W2036725530 hasAuthorship W2036725530A5077467792 @default.
• W2036725530 hasAuthorship W2036725530A5078080372 @default.
• W2036725530 hasConcept C113246987 @default.
• W2036725530 hasConcept C12554922 @default.
• W2036725530 hasConcept C135903288 @default.
• W2036725530 hasConcept C153461711 @default.
• W2036725530 hasConcept C178790620 @default.
• W2036725530 hasConcept C181199279 @default.
• W2036725530 hasConcept C185592680 @default.
• W2036725530 hasConcept C202751555 @default.
• W2036725530 hasConcept C2777022698 @default.
• W2036725530 hasConcept C2778470358 @default.
• W2036725530 hasConcept C2779066535 @default.
• W2036725530 hasConcept C2779530196 @default.
• W2036725530 hasConcept C4141045 @default.
• W2036725530 hasConcept C519063684 @default.
• W2036725530 hasConcept C55493867 @default.
• W2036725530 hasConcept C79879829 @default.
• W2036725530 hasConcept C86803240 @default.
• W2036725530 hasConcept C95444343 @default.
• W2036725530 hasConcept C98539663 @default.
• W2036725530 hasConceptScore W2036725530C113246987 @default.
• W2036725530 hasConceptScore W2036725530C12554922 @default.
• W2036725530 hasConceptScore W2036725530C135903288 @default.
• W2036725530 hasConceptScore W2036725530C153461711 @default.
• W2036725530 hasConceptScore W2036725530C178790620 @default.
• W2036725530 hasConceptScore W2036725530C181199279 @default.
• W2036725530 hasConceptScore W2036725530C185592680 @default.
• W2036725530 hasConceptScore W2036725530C202751555 @default.
• W2036725530 hasConceptScore W2036725530C2777022698 @default.
• W2036725530 hasConceptScore W2036725530C2778470358 @default.
• W2036725530 hasConceptScore W2036725530C2779066535 @default.
• W2036725530 hasConceptScore W2036725530C2779530196 @default.
• W2036725530 hasConceptScore W2036725530C4141045 @default.
• W2036725530 hasConceptScore W2036725530C519063684 @default.
• W2036725530 hasConceptScore W2036725530C55493867 @default.
• W2036725530 hasConceptScore W2036725530C79879829 @default.
• W2036725530 hasConceptScore W2036725530C86803240 @default.
• W2036725530 hasConceptScore W2036725530C95444343 @default.
• W2036725530 hasConceptScore W2036725530C98539663 @default.
• W2036725530 hasIssue "4" @default.
• W2036725530 hasLocation W20367255301 @default.
• W2036725530 hasLocation W20367255302 @default.
• W2036725530 hasOpenAccess W2036725530 @default.
• W2036725530 hasPrimaryLocation W20367255301 @default.
• W2036725530 hasRelatedWork W1965024517 @default.
• W2036725530 hasRelatedWork W1994155010 @default.

• next